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Title

 

 

 

 

 

Phylogenetic analysis of chloroplast matK gene from Zingiberaceae for plant DNA barcoding

 

Authors

 

Dhivya Selvaraj1, Rajeev Kumar Sarma1 and Ramalingam Sathishkumar1, *

 

Affiliation

 

1Department of Biotechnology, Bharathiar University, Coimbatore, India

 

Email

 

sathishkumar_ram@hotmail.com; * Corresponding author

 

Article Type

 

Hypothesis

 

Date

 

received May 08, 2008; revised June 18, 2008; accepted July 20, 2008; published September 08, 2008

 

Abstract

 

 

 

 

 

 

 

 

 

MaturaseK gene (MatK) of chloroplast is highly conserved in plant systematics which is involved in Group II intron splicing. The size of the gene is 1500 bp in length, located with in the intron of trnK. In the present study, matK gene from Zingiberaceae was taken for the analysis of variants, parsimony site, patterns, transition/tranversion rates and phylogeny. The family of Zingiberaceae comprises 47 genera with medicinal values. The matK gene sequence have been obtained from genbank and used for the analysis. The sequence alignments were performed by Clustal X, transition/transversion rates were predicted by MEGA and phylogenetic analyses were carried out by PHYLIP package. The result indicates that the Zingiberaceae genus Afromonum, Alpinia, Globba, Curcuma and Zingiber shows polyphylogeny. The overall variants between the species are 24% and transition/transversion rate is 1.54. Phylogenetic tree was designed to identify the ideal regions that could be used for defining the inter and interageneric relationships. From this study it could be concluded that the matK gene is a good candidate for DNA barcoding of plant family Zingiberaceae.

 

Keywords

 

maturaseK; Zingiberaceae; transition/transversion; phylogenetic tree; consistency index; retention index; MEGA; PHYLIP

 

Citation

 

Selvaraj et al., Bioinformation 3(1): 24-27 (2008)

 

Edited by

 

P. Kangueane

 

ISSN

 

0973-2063

 

Publisher

 

Biomedical Informatics

 

License

 

 

This is an Open Access article which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited. This is distributed under the terms of the Creative Commons Attribution License.