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Title

 

 

 

 

 

Identification of Comamonas species using 16S rRNA gene sequence

 

Authors

 

Vimlesh Yadav1, Satya Prakash1, Shipra Srivastava1, Praveen Chandra Verma2, 3, Vijayta Gupta3, Vaishali Basu3 and Anil Kumar Rawat1,*

 

Affiliation

 

1Biotechnology and Bioinformatics Division, BIOBRAINZ, 566/29 J, Jai Prakash Nagar, Alambagh, Lucknow 226005, U.P., India; 2Department of Biotechnology, Madhav Institute of Technology & Science, Gwaliar (MP), 3Present address: National Botanical Research Institute, Rana Pratap Marg, Lucknow, U.P. India

 

Email

akrbz@rediffmail.com

 

Phone

91- 0522 4042284

 

Article Type

 

Hypothesis

 

Date

 

received March 05, 2009; accepted May 16, 2009; published June 13, 2009

 

Abstract

 

A bacterial strain Bz02 was isolated from a water sample collected from river Gomti at the Indian city of Lucknow. We characterized the strain using 16S rRNA sequence. Phylogenetic analysis showed that the strain formed a monophyletic clade with members of the genus Comamonas. The closest phylogenetic relative was Comamonas testosteroni with 95% 16S rRNA gene sequence similarity. It is proposed that the identified strain Bz02 be assigned as the type strain of a species of the genus Comamonas (Comamonas sp Bz02) based on 16S rRNA gene sequence search in Ribosomal Database Project, small subunit rRNA and large subunit rRNA databases together with the phylogenetic tree analysis. The sequence is deposted in GenBank with the accession number FJ211417.

 

Keywords

16S ribosomal RNA gene; Comamonas; PCR; Phylogenetic analysis; DNA isolation; DNA Sequencing

 

Citation

Yadav et al, Bioinformation 3(9): 381-383 (2009)

 

Edited by

P. Kangueane

 

ISSN

0973-2063

 

Publisher

Biomedical Informatics

 

License

 

 

This is an Open Access article which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited. This is distributed under the terms of the Creative Commons Attribution License.