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Title

 

 

 

 

 

Identification and modeling of a drug target for Clostridium perfringens SM101

Authors

Gagan Chhabra#, Pramila Sharma#, Avishek Anant, Sachin Deshmukh, Himani Kaushik, Keshav Gopal, Nutan Srivastava, Neeraj Sharma, Lalit C. Garg*

 

Affiliation

 

Gene Regulation Laboratory, National Institute of Immunology, Aruna Asaf Ali Marg, New Delhi Ė 110067, India

 

Email

 

lalit@nii.res.in

Article Type

 

Hypothesis

Date

 

Received December 14, 2009; Revised December 26, 2009; Accepted December 28, 2009; Published January 17, 2010

 

Abstract

In the present study, comparative genome analysis between Clostridium perfringens and the human genome was carried out to identify genes that are essential for the pathogenís survival, and non-homologous to the genes of human host, that can be used as potential drug targets. The study resulted in the identification of 426 such genes. The number of these potential drug targets thus identified is significantly lower than the genomeís protein coding capacity (2558 protein coding genes). The 426 genes of C. perfringens were further analyzed for overall similarities with the essential genes of 14 different bacterial species present in Database of Essential Genes (DEG). Our results show that there are only 5 essential genes of C. perfringens that exhibit similarity with 12 species of the 14 different bacterial species present in DEG database. Of these, 1 gene was similar in 12 species and 4 genes were similar in 11 species. Thus, the study opens a new avenue for the development of potential drugs against the highly pathogenic bacterium. Further, by selecting these essential genes of C. perfringens, which are common and essential for other pathogenic microbial species, a broad spectrum anti-microbial drug can be developed. As a case study, we have built a homology model of one of the potential drug targets, ABC transporter-ATP binding protein, which can be employed for in silico docking studies by suitable inhibitors.

 

Keywords

Clostridium perfringens, DEG, Essential genes, Drug targets, Broad-spectrum anti microbial drug.

 

Citation

 

Chhabra et al., Bioinformation 4(7): 278-289 (2010)

Edited by

 

P. Kangueane

 

ISSN

 

0973-2063

 

Publisher

 

Biomedical Informatics

Copyright

 

Publisher

 

Copyright Transfer Agreement

 

The authors of published articles in Bioinformation automatically transfer the copyright to the publisher upon formal acceptance. However, the authors reserve right to use the information contained in the article for non commercial purposes.

 

License

 

 

This is an open-access article, which permits unrestricted use, distribution, and reproduction in any medium, for non-commercial purposes, provided the original author and source are credited.