Title

Early diagnosis of systemic lupus erythmatosus using ANN models of dsDNA binding antibody sequence data

Authors

Mohamad Hasan Bahari¹, Mahmoud Mahmoudi², Asad Azemi³, Mir Mojtaba Mirsalehi⁴, Morteza Khademi⁵*

Affiliation

¹Katholieke Universiteit Leuven/ESAT, Leuven, Belgium. ² Immunology Research Center, Mashhad University of Medical Science, Mashhad, Iran. ³ Penn State University /Engineering Department, Delaware, USA. ⁴ Ferdowsi University of Mashhad/Electrical Engineering Department, Mashhad, Iran. ⁵ Ferdowsi University of Mashhad/Electrical Engineering Department, Mashhad, Iran.

E-mail*

¹ m.h.bahari@ieee.org ² mahmoudim@mums.ac.ir ³ Azemi@psu.edu ⁴ mirsalehi@um.ac.ir ⁵ khademi@um.ac.ir

Article Type

Hypothesis

Date

received  March 28, 2010; revised April 30, 2010; accepted  June 8, 2010; published online July06, 2010

In this paper a new method based on artificial neural networks (ANN), is introduced for identifying pathogenic antibodies in Systemic Lupus Erythmatosus (SLE). dsDNA binding antibodies have been implicated in the pathogenesis of this autoimmune disease. In order to identify these dsDNA binding antibodies, the protein sequences of 42 dsDNA binding and 608 non-dsDNA binding antibodies were extracted from Kabat database and encoded using a physicochemical property of their amino acids namely Hydrophilicity. Encoded antibodies were used as the training patterns of a general regression neural network (GRNN). Simulation results show that the accuracy of proposed method in recognizing dsDNA binding antibodies is 83.2%. We have also investigated the roles of the light and heavy chains of anti-dsDNA antibodies in binding to DNA. Simulation results concur with the published experimental findings that in binding to DNA, the heavy chain of anti-dsDNA is more important than their light chain. 

Keywords

Anti-dsDNA; Antibody; General Regression Neural Network (GRNN); Systemic Lupus Erythematosus

Citation

Bahari et al, Bioinformation 5(2): 58-61 (2010)

Edited by

P. Kangueane

ISSN

0973-2063

Publisher

License