Title

Authors

Affiliation

Molecular Taxonomy Laboratory, Department of Zoology, University Road, C. C. S. University, Meerut (UP), 250004, India.

Email

anshu8282@rediffmail.com; *Corresponding author

Article Type

Hypothesis

Date

Received August 31, 2012; Accepted September 03, 2012; Published September 21, 2012

The present study was performed to identify and validate monogenean species from different piscine hosts using molecular tools. Nine species of freshwater monogeneans were collected from gills and skin of freshwater fishes at Hastinapur, Meerut, India. After microscopic examination, molecular analysis was performed utilizing 28S gene marker. Phylogenetic analysis indicated the validation and systematic position of these nine different monogeneans belongs to the Dactylogyridae and Gyrodactylidae families. The findings also confirm that the 28S rDNA sequence is highly conserved and may prove to be useful in taxonomic studies of parasitic platyhelminthes. Besides this, the study is also supplemented by molecular morphometrics that is based on 28S secondary structure homologies of nine monogenean species. The data indicate that 28S motifs i.e., = 50bp in size can also be considered a promising tool for monogenean species identification and their validation.

Keywords

Monogenea, India, 28S rDNA, Secondary structure prediction, motif, phylogeny

Citation

Chaudhary & Singh, Bioinformation 8(18): 862-869 (2012)
 

Edited by

P Kangueane

ISSN

0973-2063

Publisher

Biomedical Informatics

License

This is an Open Access article which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited. This is distributed under the terms of the Creative Commons Attribution License.