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Title

Understanding mechanism of in vitro maturation, fertilization and culture of sheep embryoes through in silico analysis

 

Authors

Dulam Sreenivas1, Dowluru SVGK Kaladhar2*, A Palni Samy3 & R Sangeeth Kumar3

 

Affiliation

1Chennai Fertility Centre, Chennai, 2GITAM University, Visakhapatnam, 3Dept. of animal Biotechnology, Tamilnadu Veterinary, and Animal Sciences University, Chennai.

 

Email

dr.dowluru@gmail.com; *Corresponding author

 

Article Type

Hypothesis

 

Date

Received October 17, 2012; Accepted October 18, 2012; Published October 31, 2012

 

Abstract

Protein interations are presently required to understand the mechanisms of in vitro maturation, fertilization and culture of sheep embryoes through in silico analysis. The present work has been conducted on TCM-199 supplemented with epidermal growth factor (EGF), fetal bovine serum (FBS) or wheat peptones The maturation rate of oocyte was significantly higher in the FBS supplemented group when compared with BSA and wheat peptone supplemented groups. The in silico protein interaction studies has shown that the proteins EGFR (epidermal growth factor receptor), CCK (cholecystokinin)- a peptide hormone, Alb a serum albumin, ESR- estrogen receptor 1, TGFA- transforming growth factor, STAT- signal transducer and FN1- fibronectin 1 has direct interaction and produces cell growth in in vitro culture. Alb is directly activates EGF and promotes MAPK3 that mediates diverse biological functions such as cell growth, adhesion and proliferation. Alb may also involve in stress response signalling and may be in cell cycle control.

 

Keywords

in vitro embryo development, sheep, protein interaction

 

Citation

Sreenivas et al. Bioinformation 8(21): 1030-1034 (2012)
 

Edited by

P Kangueane

 

ISSN

0973-2063

 

Publisher

Biomedical Informatics

 

License

This is an Open Access article which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited. This is distributed under the terms of the Creative Commons Attribution License.