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Title

Screening of inhibitors for S130G inhibitor resistant mutants of TEM type beta-lactamase

 

Authors

Mohd Hassan Baig1, Mohd Danishuddin2, Saif Khan1 & Asad U Khan2*

 

Affiliation

1Department of Biotechnology, Microbiology and Bioinformatics, Integral University, Lucknow-226026, India; 2Interdisciplinary Biotechnology Unit, Aligarh Muslim University Aligarh, India.

 

Email

asad.k@rediffmail.com; *Corresponding author

 

Article Type

Hypothesis

 

Date

Received October 21, 2012; Accepted October 26, 2012; Published December 08, 2012

 

Abstract

Bacteria are remarkably adaptable organisms that acquire an almost limitless competence to survive under unpleasant conditions. The drastic emergence of antibiotic resistance among -Lactamases is the most serious threat to hospitals and nosocomial settings. -lactam inhibitors came into existence in order to overcome the problem of antibibiotic resistance in bacteria. The emergence of inhibitor resistant mutants has raised the alarms. In this study we have used structured based virtual screening approach and have screened out some inhibitors against S130G TEM mutant. All the compounds were tested in presence and absence of conserved active site water molecules. These compounds were found be showing much higher efficacy than known -lactamase inhibitors. Amino acids G130, S70, N132, G130, Y105 and V216 were found crucial for the interaction of inhibitors within the active site.

 

Citation

Baig et al. Bioinformation 8(24): 1225-1229 (2012)

Edited by

P Kangueane

 

ISSN

0973-2063

 

Publisher

Biomedical Informatics

 

License

This is an Open Access article which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited. This is distributed under the terms of the Creative Commons Attribution License.