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Title

Isolation and In-silico characterization of Peroxidase isoenzymes from Wheat (Triticum aestivum) against Karnal Bunt (Tilletia indica)

 

Authors

Shalini Purwar*, Ankit Gupta, Geetika Vajpayee & Shanthy Sundaram

 

Affiliation

Center for Biotechnology University of Allahabad, Allahabad

 

Email

purwarshalini@gmail.com; *Corresponding author

 

Article Type

Hypothesis

 

Date

Received January 16, 2014; Accepted January 26, 2014; Published February 19, 2014

 

Abstract

To investigate the role of Peroxidase and its physiological significance under Karnal Bunt (KB) were determined in resistant (HD-29) and susceptible genotype (WH-542) of wheat during different developmental stages. The enzymes were expressed constitutively in both the susceptible and resistant genotype. In gel assay and differential expression analysis of POD was significantly higher (p >0.05) in Sv and S2, than the S1 and S3 stages. In silico analysis of Peroxidase for eg. physico-chemical properties, secondary structural features and phylogenetic classification for comparative analysis. Motif and Domain analysis of Peroxidase by MEME, to be important for the biological functions, and studies of evolution. Our results clearly indicate that the enhanced expression of POD at the WS2 stage, which reinforces its role in stage dependent immunity against Karnal bunt and role of POD metabolism provides genotype and stage dependant structural barrier resistance in wheat against KB.

 

Keywords

Peroxidase (POD), Karnal Bunt, Triticum, insilico analysis, Fungus

 

Citation

Purwar  et al. Bioinformation 10(2): 087-093 (2014)
 

Edited by

P Kangueane

 

ISSN

0973-2063

 

Publisher

Biomedical Informatics

 

License

This is an Open Access article which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited. This is distributed under the terms of the Creative Commons Attribution License.