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Analysis of salt-bridges in prolyl oligopeptidase from Pyrococcus furiosus and Homo sapiens



Amal Kumar Bandyopadhyay*,1, Rifat Nawaz Ul Islam2,$, Debanjan Mitra1,$, Sahini Banerjee3,$ and
Arunava Goswami3



1Department of Biotechnology, The University of Burdwan, Burdwan, West Bengal, India; 2Department of Zoology, The University of Burdwan, Burdwan, West Bengal, India; 3Department of Biological Sciences, ISI, Kolkata, West Bengal, India; $equal contribution;



Amal Kumar Bandyopadhyay E-mail:; *corresponding author


Article Type

Research Article



Received January 9, 2019; Revised March 9, 2019; Accepted March 11, 2019; Published March 15, 2019



Hyper thermophilic archaea not only tolerate high temperature but also operate its biochemical machineries, normally under these conditions. However, the structural signatures in proteins that answer for the hyper thermo-stability relative to its mesophilic homologue remains poorly understood. We present a comparative analyses of sequences, structures and salt-bridges of prolyl-oligopeptidase from Pyrococcus furiosus (pfPOP - PDB ID: 5T88) and human (huPOP - PDB ID: 3DDU). A similar level of hydrophobic and hydrophilic residues in pfPOP and huPOP is observed. A low level of interactions between shell-waters and atom-types in pfPOP indicated hyper thermo-philic
features are negligible. Salt-bridge-forming-residues (sbfrs) are high in pfPOP core and surface (pfPOP). Increased sbfrs largely indicate specific-electrostatic is important for thermo stability in pfPOP. Four classes of sbfrs are found namely positionally non-conservative (PNCS), conservative (PCS), unchanged (PU) and interchanged (PIC) type of substitutions. PNCS-sbfrs constitutes 28% and it is associated with the topology of pfPOP at high temperature. PCS helps to increase the salt-bridge population. It is also found that PU maintains similar salt-bridges at the active site and other binding sites while PIC abolishes mesophilic patterns.



Thermo stability; salt bridge; salt bridge design; electrostatics; prolyl oligo peptidase



Bandyopadhyay et al. Bioinformation 15(3): 214-225 (2019)


Edited by

P Kangueane






Biomedical Informatics



This is an Open Access article which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited. This is distributed under the terms of the Creative Commons Attribution License.