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Title

Genetic association of MBL-2 gene polymorphisms with Filarial chyluria

 

Authors

1Shriya Pant, 1Apul Goel*, 1Pravin Kumar Gangwar, 2Jyotsna Agarwal, 3Arvind Kumar Singh, 1Satya
Narayan Sankhwar; 3Prashant Gupta

 

Affiliation

1Department of Urology, King George's Medical University, Lucknow, UP, India; 2Department of Microbiology, Dr. Ram Manohar Lohia Institute of Medical Sciences, India; 3Department of Microbiology, King George's Medical University, Lucknow, UP, India

 

Email

Apul Goel Email:drapul.goel@gmail.com; *Corresponding author

 

Article Type

Research Article

 

Date

Received November 14, 2019; Revised November 28, 2019; Accepted December 7, 2019; Published December 10, 2019

 

Abstract

Lymphatic filariasis has become a significant public health issue in North India. The association of polymorphisms in MBL2 gene with filarial chyluria (FC) is evaluated in the North Indian patients for the first time. Hence, a tertiary care hospital based case-control study was conducted in north India where FC is endemic. Therefore, 186 confirmed patients of FC as cases and 210 age-, sex- and residence-matched subjects as controls were enrolled for the study. Filarial etiology was confirmed using diethylcarbamazine (DEC)-provocation test, immune chromatographic test and IgG/IgM antibody test. MBL2 gene polymorphisms at codon 54 and -221 promoter region were genotyped by
PCR followed by RFLP. Wild-type, heterozygous and homozygous mutant frequencies of MBL2 genotype at the codon 54 were 57.5%, 32.8% and 9.7% in the case group and 62.9%, 30.5% and 6.7%, in controls, respectively. The same at the -221 position were 51.1%, 44.1% and 4.8% in FC patients and 44.3%, 40.0% and 15.7% in controls, respectively. Thus, results no significant association between MBL2 polymorphism at codon 54 and FC. However, polymorphism at the -221 promoter region is linked with FC with a significant odd-ratio of 0.27 (confidence interval at 95% was 0.12-0.59; p<0.001). This preliminary finding is intriguing for further confirmation using a larger study with more patients.

 

Keywords

Mannose-binding lectin; lymphatic filariasis; chyluria.

 

Citation

Pant et al. Bioinformation 15(11): 806-811 (2019)

 

Edited by

P Kangueane

 

ISSN

0973-2063

 

Publisher

Biomedical Informatics

 

License

This is an Open Access article which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited. This is distributed under the terms of the Creative Commons Attribution License.