Title
Identification of arsenic resistant bacterial isolates from semi arid coal mine tailings of Assam using 16S rRNA phylogeny
Authors
Alka Singh & Surya Prakash Dwivedi*
Affiliation
Environmental Biotechnology Lab, School of Biotechnology, IFTM University, Moradabad, Uttar Pradesh, India-244001
Surya Prakash Dwivedi - E-mail: surya.miet@gmail.com, *Corresponding author
Article Type
Research Article
Date
Received November 28, 2019; Revised December 20, 2019; Accepted December 27, 2019; Published December 31, 2019
Abstract
Rhizospheric and non rhizospheric soil samples were taken from the tailings of North Eastern coal mines of Assam state of India to isolate the arsenic resistant bacteria having the ability to transform different arsenic forms particularly the toxic As (III) and As (V) using arsenic bioremediation methods. We describe the isolation and characterization of four strains with high resistance towards arsenic. Isolates were processed and characterized using biochemical and molecular characterization techniques. The 16S ribosomal RNA gene isolated were sequenced and the sequencing data were aligned using the multiple sequencing alignment tool (MSA). The sequences of the strains formed a monophyletic clade with the members of the genus Bacillus and Rhodanobacter in the The results of phylogenetic tree. The sequence representing Bacillus subtilis showed maximum similarity with 99% 16S rRNA gene sequence similarity followed by Bacillus cereus (99%) and Rhodanobacter thiooxydans with 97% similarity. The phylogenetic tree data suggests that the isolated and identified strains is of the type strains of species belonging to the genus Bacillus and Rhodanobacter together with the 16S rRNA gene sequence in the Ribosomal Database, small subunit rRNA and large subunit rRNA databases. It is of interest to isolate, identify and characterize the highly resistant strains, which were highly resistant to arsenic from the rhizospheric and non-rhizospheric soils from the tailings of the coalmines of Assam using the 16S rRNA gene sequence phylogenetic analysis. Thus, the four arsenic resistant strains were reported using the 16S rRNA gene sequence phylogenetic analysis. The accession numbers for the sequences are MN706211, MN701565, MN706210 and MN706212. A monophyletic clade representing the members of the genus Bacillus (Bacillus subtilis and Bacillus cereus) and Rhodanobacter thiooxydans is thus documented.
Keywords
16S rRNA gene, phylogenetic, arsenic resistant bacteria, ribosomal database, coal mine tailing
Citation
Singh & Dwivedi, Bioinformation 15(12): 869-874 (2019)
Edited by
P Kangueane
ISSN
0973-2063
Publisher
License
This is an Open Access article which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited. This is distributed under the terms of the Creative Commons Attribution License.
