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Title

Comparative thermo-stability of two Rift Valley fever virus vaccine candidate 13T with a recombinant arMP-12 delta NSm21/384 protein

 

Authors

S Daouam1,2,**, Z Boumart1,**, A Elarkam1, J Hamdi1, KO Tadlaoui1, MM Ennaji2,* & MEL harraka

 

Affiliation

1Research and Development Virology, Multi-Chemical Industry, Morocco; 2Laboratory of Virology, Microbiology, Quality and Biotechnology/ETB, Faculty of Sciences & Technics Mohammedia, Morocco

 

Email

Tel: +212 661-748862; E-mail: m.ennaji@yahoo.fr; *Corresponding author; **Equal Contribution

 

Article Type

Research Article

 

Date

Submitted on March 16, 2020; Revision June 1, 2020; Accepted June 10, 2020; Published July 31, 2020

 

Abstract

Rift Valley fever (RVF) is a zoonotic, viral disease, transmitted by mosquitoes, characterized by high mortality rates in young animals. RVF is an endemic and enzootic disease in the Arabian Peninsula and Africa, causing public health and economic instability. Therefore, it is important to develop vaccines to minimize outbreaks and combat the disease. We documented the stability of the thermo-stability of live attenuated RVF CL13T and recombinant arMP-12 delta NSm21/384 vaccine candidates at different temperatures, including these vaccine viruses in liquid and lyophilized form. The study revealed that both CL13T and recombinant arMP-12 delta NSm21/384 strains were stable for more than 18 months at 4C. We show that at room temperatures (37C and 45C) the CL13T was less temperature sensitive than MP-12NSm-del in both lyophilized and liquid form. These findings are useful for the preparation of RVF vaccines that will avoid the need for a cold chain and therefore, will improve the application of the vaccines under field conditions.

 

Keywords

Stability, Clone 13T vaccine, arMP-12 delta NSm21/384 vaccine.

 

Citation

Daouam et al. Bioinformation 16(7): 547-554 (2020)

 

Edited by

P Kangueane

 

ISSN

0973-2063

 

Publisher

Biomedical Informatics

 

License

This is an Open Access article which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited. This is distributed under the terms of the Creative Commons Attribution License.