HOME   |    PDF   |   


Description of microbial diversity associated with ticks Hyalomma dromedarii (Acari: Ixodidae) isolated from camels in Hail region (Saudi Arabia) using massive sequencing of 16S rDNA



Mousa M. Alreshidi1*, Vajid N. Veettil1, Emira Noumi1,2, Rosa Del Campo3, Mejdi Snoussi1,4



1Department of Biology, College of Science, Ha’il, P.O. 2440, University of Ha’il City 2440, Saudi Arabia (M.M.A): mo.alreshidi@uoh.edu.sa, (V.N.V.): n.vajid@uoh.edu.sa. 2Laboratory of Bioressources: Integrative Biology and Recovery, High Institute of Biotechnology-University of Monastir, Monastir 5000, Tunisia (E.N.): eb.noumi@uoh.edu.sa. 3Servicio de Microbiología, Instituto Ramón y Cajal de Investigación Sanitaria (IRYCIS), Hospital Universitario Ramón y Cajal, Carretera de Colmenar, Km 9,1, 28034 - Madrid. Spain (R.D.C.): rosacampo@yahoo.com. 4Laboratory of Genetics, Biodiversity and Valorization of Bio-resources, Higher Institute of Biotechnology of Monastir, University of Monastir, Avenue Tahar Haddad, 5000 Monastir, Tunisia (M.S.): m.snoussi@uoh.edu.sa)



*Corresponding author: Dr. Mousa Alreshidi, E-mail: mo.alreshidi@uoh.edu.sa


Article Type

Research Article



Received July 3, 2020; Revised July 5, 2020; Accepted July 5, 2020; Published August 31, 2020



Ticks are blood feeder able to transmit a wide diversity of microbes including pathogens. Therefore, it is of our interest to detect the diversity of microorganisms residing within ticks using massive sequencing of 16S rDNA. In this study, 200 adult ticks were collected from healthy camels in two localities from Hail province (Saudi Arabia). The analysis showed high microbial diversity dominated by the two domains (Archaea and Bacteria) associated with Hyalomma dromedarii from both regions. Proteobacteria (61.3%) and Firmicutes (31.2%) dominated the ticks from the Al Khotha region. While, the microbiome of ticks from the Al Gayed region was dominated by Proteobacteria (81.2%) and Firmicutes (9.2%). Twenty-three families were identified in the DNA-pool from the Al Gayed region, and was dominated by Pseudomonadaceae (45.37%), and Marinobacteraceae (14.39%) families. Francisellaceae (46%), Staphylococcaceae (24.26%) dominated the microbiome of the ticks collected from Al Gayed region. Thus, the genera Pseudomonas, Francisella, Proteus, Marinobacter, Glutamicibacter, Pedobacter, and Staphylococcus are largely distributed in the two identified microbiomes. This study concluded that ticks collected from the studied localities contained a wide range of microbial communities. These data have a great veterinary and medical importance in near future.



Hyalomma dromedarii, 16S RNA, metagenomic, camels, Hail region



Alreshidi et al. Bioinformation 16(8): 602-610 (2020)


Edited by

P Kangueane






Biomedical Informatics



This is an Open Access article which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited. This is distributed under the terms of the Creative Commons Attribution License.