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Insights on seed abortion (endosperm and embryo development failure) from the transcriptome analysis of the wild type plant species Paeonia lutea



Shanshan Zhang1,2, Yongning Cui1,2, Yuanhui Li1,2, Fan Zhao1,2, Yuanrong Li1,2, Xiuli Zeng1,2,*



1The Ministry of Agriculture of Qinhai-Tibet plateau Fruit Trees Scientific Observation Test Station, Lhasa, Tibet 850032, China; 2Institute of Vegetables, Tibet Academy of Agricultural and Animal Husbandry Sciences, Lhasa, Tibet 850002, China



E-mail: nkyzss0123@126.com; *Corressponding author


Article Type

Research Article



Submitted on January 13, 2020; Revision July 29, 2020; Accepted July 31, 2020; Published August 31, 2020



Paeonia lutea is a wild peony (an endangered flowering plant species) found in China. Seed abortion (endosperm and embryo development failure) is linked to several endangered plant species. Therefore, it is of interest to complete a comparative analysis of transcriptome between the normal active seeds (Population A) and the endangered abortion seeds (Population H). Data from GO assignments of differentially expressed genes (DEGs) shows that “metabolic process”, “binding”, “cellular process”, “catalytic activity”, “cell” and “cell part” are commonly prevalent in these popuations. DEGs between the populations are found to be connected with metabolic pathways, biosynthesis of secondary metabolites, purine metabolism and ribosome. We used quantitative RT-PCR to validate 16 DEGs associated with these populations. It is found that histone genes and proline-rich extensin genes are predominant in the common groups. Histone genes (H2A, H2B, H3, H4 and linker histone H1) show 3 to 4 folds log2FC higher expession in population A than in population H in stage I unlike in stage II and III. Increased activity of proline-rich extensin genes in population A than in population H corresponding to seed abortion in the later population is implied. These preliminary data from the transcriptome analysis of the wild type plant species Paeonia lutea provide valuable insights on seed abortion.



Paeonia lutea, Seed abortion, Transciptome



Zhang et al. Bioinformation 16(8): 638-651 (2020)


Edited by

P Kangueane






Biomedical Informatics



This is an Open Access article which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited. This is distributed under the terms of the Creative Commons Attribution License.