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Neurobehavioral responses in swiss albino mice induced by an aqueous leaf extract from a medicinal plant named Heliotropium incanum ruiz & pav.



H. Ashalata Singha1, MahuyaSengupta2, Meenakshi Bawari1*



1Department of Life Science and Bioinformatics, Assam University, Silchar, Assam, India 788011, Email: ashalata.singha@gmail.com; 2Department of Biotechnology,



Email: senguptamahuya35@gmail.com; Phone: +91 9435171903; Meenakshi Bawari* - Email: mbawari.aus@gmail.com; *Corresponding Author


Article Type

Research Article


Received July 23, 2020; Revised July 30, 2020; Accepted July 31, 2020; Published September 30, 2020



It is of interest to examine the adverse neuro-behavioural responses on mice treated with the aqueous crude extract of Heliotropium incanum (AEHI), which were evaluated using various behavioral paradigms. On the basis of median lethal dose value, doses of AEHI were chosen to be 150mg/kg and 440mg/kg for further experiment. Four groups comprising of five mice each were divided for the 14 days experiment. Group I, the control group, received distilled water; Group II and III received AEHI (150 mg/kg body weight and 440 mg/kg body weight) respectively; Group IV received standard drugs, Diazepam/Fluoxetine, administered orally. On administration of AEHI, it was revealed that dose 440 mg/kg showed less exploration activity in the hole board test; decrease in the number of squares crossed in locomotory test, time period in the open arm in the plus maze test was significantly reduced and the immobility time was significantly extended in comparison to control and standard drugs. The microscopic study of brain revealed damaged hippocampus along with nerve cells degeneration. Consequently, the results concluded that the outcome of the AEHI produced evidences for the anxiogenic activity in mice.



neurobehavioral changes, medicinal plant, anxiogenic, Heliotropium incanum, toxic effect



Singha et al. Bioinformation 16(9): 679-687 (2020)


Edited by

P Kangueane






Biomedical Informatics



This is an Open Access article which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited. This is distributed under the terms of the Creative Commons Attribution License.