Title
Cytotoxic potentials of silibinin assisted silver nanoparticles on human colorectal HT-29 cancer cells
Authors
Kiren Jackson1, Ezhilarasan Devaraj*1, Thangavelu Lakshmi1, Shanmugam Rajeshkumar1, Kamal Dua2,3, Dinesh Kumar Chellappan4, & Subramanian Raghunandhakumar1
Affiliation
1Department of Pharmacology, Saveetha Dental College, Saveetha Institute of Medical and Technical Sciences (SIMATS), Chennai, Tamil Nadu, India 600 077; 2Discipline of Pharmacy, Graduate School of Health, University of Technology Sydney, Sydney, NSW 2007, Australia; 3School of Biomedical Sciences and Pharmacy, University of Newcastle, Newcastle, NSW 2308, Australia; 4Department of Life Sciences, School of Pharmacy, International Medical University, Bukit Jalil 57000, Kuala Lumpur, Malaysia
Dr. D. Ezhilarasan - E- mail: ezhild@gmail.com; Phone: (Off) 044-26801580-87; Fax: +91 44 26800892; Mobile: +91 9944008201; *Corresponding author
Article Type
Research Article
Date
Received September 10, 2020; Revision September 26, 2020; Accepted September 26, 2020; Published November 30, 2020
Abstract
It is of interest to study the cytotoxicity of silibinin assisted silver nanoparticles in human colorectal (HT-29) cancer cells. Silver nanoparticles were synthesized using silibinin as a reducing agent. The synthesized silibinin assisted silver nanoparticles (SSNPs) were characterized and analyzed using a transmission electron microscope and spectrophotometer. The SSNPs synthesized in this study are spherical and their size ranges from 10 to 80 nm. HT-29 cells were treated with different concentrations (2, 4, 6, 8 and 10 ng/mL) of SSNPs and cytotoxicity was evaluated. The apoptosis was using flow cytometry. p53 protein expression using western blot. SSNPs are induced a decrease in viability and increased concentration-dependent cytotoxicity in HT-29 cells. SSNPs treatment also caused apoptosis-related morphological changes. SSNPs treatments at 8 and 16 ng/ml showed a prominent apoptotic change i.e., 70.3% and 83.6% respectively, and decreased viability of HT-29 cells 20% and 11.2% respectively as compared to control cells. SSNPs treatments induced p53 expression in HT-29 cells. Data shows that SSNPs have the potential to induce apoptosis in colorectal cancer cells. This provides insights for the further evaluation of SSNPs in fighting colon cancer.
Keywords
Colorectal cancer, silibinin, silver nanoparticles, apoptosis
Citation
Jackson et al. Bioinformation 16(11): 817-827 (2020)
Edited by
P Kangueane
ISSN
0973-2063
Publisher
License
This is an Open Access article which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited. This is distributed under the terms of the Creative Commons Attribution License.
