Title

Anticancer effects and lysosomal acidification in A549 cells by astaxanthin from Haematococcus lacustris

Authors

K. Ramamoorthy¹, Subramanian Raghunandhakumar^2*, RS. Anand³, A. Paramasivam⁴, S. Kamaraj⁵, S Nagaraj¹, Devaraj Ezhilarasan^2,4, Thangavelu Lakshmi², Kamal Dua^6,7, Dinesh Kumar Chellappan⁸, Ashokkumar Veeramuthu⁹

Affiliation

¹Centre for Advanced Studies in Botany, University of Madras (Guindy Campus), Chennai, India-600 025; ²Department of Pharmacology, Saveetha Dental College, Saveetha University, Chennai, India; ³Centre for Biotechnology, Anna University, Chennai - 600 025; ⁴Biomedical Research Unit and Laboratory Animal Centre-Dental Research Cell, Saveetha Dental College, Saveetha University, Chennai, India; ⁵Department of Biotechnology, Periyar University (PG Extension Centre), Dharmapuri – 636701; ⁶Discipline of Pharmacy, Graduate School of Health, University of Technology Sydney, Ultimo, NSW, 2007, Australia; ⁷School of Biomedical Sciences and Pharmacy, University of Newcastle, Newcastle NSW 2308, Australia; ⁸Department of Life sciences, School of Pharmacy, International Medical University, Bukit Jalil, 57000,Kualalumpur, Malaysia; ⁹Department of Chemical Technology, Chulalongkorn University, Bangkok,Thailand

Email

Dr. S. Raghunandhakumar, E-mail: raghunandhakumar@gmail.com *Corresponding Author.

Article Type

Research Article

Date

Received October 7, 2020; Revised October 26, 2020; Accepted October 26, 2020; Published November 30, 2020

Astaxanthin (AXN) is known to have health benefits by epidemiological studies. Therefore, it is of interest to assess the effect of AXN (derived from indigenous unicellular green alga Haematococcus lacustris) to modulate cell cycle arrest, lysosomal acidification and eventually apoptosis using in vitro in A549 lung cancer cells. Natural extracts of astaxanthin were obtained by standardized methods as reported earlier and characterized by standard HPLC and MS. Treatment of A549 cells with AXN (purified fraction) showed significant reduction in cell viability (about 50%) as compared to crude extract at 50µM concentration. Thus, we show the anticancer effects and lysosomal acidification in A549 cells by Astaxanthin from Haematococcus lacustris for further consideration. Together, our results demonstrated the anticancer potential of AXN from Haematococcus lacustris, which is found to be mediated via its ability to induce cell cycle arrest, lysosomal acidification and apoptotic induction.

Keywords

Astaxanthin; Haematococcus lacustris; A549 cells; Apoptosis; Lysosomal acidification.

Citation

Ramamoorthy et al. Bioinformation 16(11): 965-973 (2020)

Edited by

P Kangueane

ISSN

0973-2063

Publisher

Biomedical Informatics

License

This is an Open Access article which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited. This is distributed under the terms of the Creative Commons Attribution License.