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Title

 Insights from the cDNA and EST analysis of Antrodia cinnamomea

Authors

 Jing Li1, Yanling Liu1, Richard Yankey1, 2, Dongmei Lin1 & Zhanxi Lin1,*

 

Affiliation

1National Engineering Research Center of JUNCAO Technology, Fujian Agriculture and orestry University, Fujian, Fuzhou 350002, PR China; 2Life Science College, Fujian Agriculture and Forestry University, Fujian, Fuzhou 350002, PR China; *Corresponding author

 

Email

 Zhanxi Lin – Email ID: lzxjuncao@163.com;

 

Article Type

 Research Article

 

Date

 Received July 21, 2020; Revised December 31, 2020; Accepted January 26, 2020, Published January 31, 2021

 

Abstract

It is of interest to document the insights gleaned from the cDNA and EST analysis of Antrodia cinnamomea (a fungal species). Hence a library of sequences was constructed and analysed using standard procedures to gain new insights. Therefore, 65 ESTs, with size ranging from 300-2000 bp, were constructed. This included 46 ESTs with definite annotation, 18 ESTs were hypothetical and 1 new protein derived from BLAST analysis. We assigned 227 Gene Ontology terms linked to cell composition, transport, catalytic activity, and regulation functions in these sequences. Moreover, 56 matching genes were found in 8 Kyoto Encyclopedia of Genes and Genomes pathways. Data also showed 271 SSRs from Antrodia cinnamomea ESTs with an occurrence frequency of 96.82%. The STRING data analysis showed 29 genes encoded enzymes highly involved in protein-to-protein interactions linked to expression of regulation function. Thus, we documented some insights from the cDNA and EST analysis of Antrodia cinnamomea for further data mining.

 

Keywords

 Antrodia cinnamomea; genome wide analysis; cDNA; EST; GO functional annotation;

 

Citation

 Li et al. Bioinformation 17(1): 109-118 (2021)

 

Edited by

P Kangueane

 

ISSN

0973-2063

 

Publisher

Biomedical Informatics

 

License

This is an Open Access article which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited. This is distributed under the terms of the Creative Commons Attribution License.