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Functional validation of miRNA target genes in abiotic stress in Hippophae salicifolia


Parneeta Chaudhary & Prakash Chand Sharma*



University School of Biotechnology, Guru Gobind Singh Indraprastha University, New Delhi, India; *Corresponding author: Prakash Chand Sharma



Parneeta Chaudhary - Email: parnitachaudhary@gmail.com & parneeta.02116091117@ipu.ac.in Prakash Chand Sharma - E-mail: prof.pcsharma@gmail.com & prof.pcsharma@ipu.ac.in


Article Type

Research Article



Received November 28, 2021; Revised December 21, 2021; Accepted December 21, 2021, Published January 31, 2022



miRNAs are non-coding, single-stranded RNAs and are known to regulate the expression of genes during the post-transcription process. Seabuckthorn (Hippophae sp.; Elaeagnaceae) plant grows in different regions in harsh environmental conditions and is tolerant to various abiotic stress prevailing in the Indian Himalayas. Therefore, it is of interest to document the functional assignment of miRNA target genes to abiotic stress in Hippophae salicifolia using available bioinformatics tools. We identified eleven miRNA target genes in the seabuckthorn transcriptome. The expression analysis of these miRNA target genes provides important information about the regulation of stress-responsive defense mechanisms in seabuckthorn. Understanding of the role of these putative miRNAs and their target genes in cold and heat tolerance provides insights to determine the potential targets for the exploitation towards the development of stress-tolerant crop plants.



Seabuckthorn, Hippophae salicifolia, miRNA, gene expression, abiotic stress.



Chaudhary & Sharma, Bioinformation 18(1): 61-65 (2022)


Edited by

P Kangueane






Biomedical Informatics



This is an Open Access article which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited. This is distributed under the terms of the Creative Commons Attribution License.