Title
Effect of β-sitosterol on insulin resistance & protein expression of insulin signalling molecules in quadriceps muscle of high fat diet-induced type-2 diabetic rats
Authors
Gayathri Sekar1,2, G Merlin3, Madhan Krishnan4†, Shyamaladevi Babu4†, K Padmini2, Konduru Pushpa5 & S Mangai1*
Affiliation
1PG & Research Department, Department of Biochemistry, Bhaktavatsalam Memorial College for Women, Korattur chennai-600080, Tamilnadu, India; 2Department of pathology, Biosciences research Foundation, Chennai, Tamilnadu, India; 3Department of Biostatistics, ACS Medical College and Hospital, Velappanchavadi, Chennai 600077, Tamilnadu, India; 4Faculty of Allied Health Sciences, Chettinad Hospital and Research Institute, Chettinad Academy of Research and Education, Kelambakkam-603103, Tamilnadu, India; 5RCC Laboratories India Private Limited, Chennai, Tamilnadu, India; *Corresponding author
Gayathrisekar –E-mail: gayathrisekar.2108@gmail.com
G Merlin- E-mail: merlin.commed@acsmch.ac.in
Madhan Krishnan- E-mail: kmadhan91@gmail.com
Shyamaladevi Babu- E-mail: shyamdevi06@gmail.com
K Padmini- E-mail: kpadmene@gmail.com
Konduru Pushpa- E-mail: konduruPushpa.bt@gmail.com
S Mangai* - E-mail: mangai31@yahoo.co.in
Article Type
Research Article
Date
Received November 1, 2022; Revised November 29, 2022; Accepted November 30, 2022, Published November 30, 2022
Abstract
Changes in life style, such as high-calorie diet intake and lack of exercise, have increased the global prevalence of obesity and diabetes. The major pathophysiological event contributing to the development of type 2 diabetes mellitus is the target tissues to insulin action. Currently available drugs are unsuccessful for the treatment of type 2 diabetes due to their unwanted side effects. Hence, search drugs, from plant sources. β-sitosterol is plant sterols with structurally almost like that of cholesterol. It is widely present in various medicinal plants. It has been reported to elicit multitude of bioactivities including anti-lipidemic and anti-hyperglycemic activity. However, specific effect of β-sitosterol on insulin signalling molecules of quadriceps muscle remains unclear. Hence, the study aimed to assess the beneficial role of β-sitosterol on the expression of insulin signalling molecules in quadriceps muscle of high fat diet and sucrose-induced type‑2 diabetic rats. The oral effective dose of β-sitosterol (25 mg/kg body weight) was given once each day until the end of the study (30 days post‑induction of diabetes) to HFD- fed diabetic rats. At the end of the experimental period, fasting blood sugar (FBG), oral glucose (OGTT) and insulin tolerances (IT), Tissue glycogen, Serum insulin, Homeostasis Model Assessment for Insulin Resistance (HOMA-IR) and Quantitative Insulin Sensitivity Check Index (QUICKI), as well as the levels of insulin signalling molecules like insulin receptor (IR), glucose transporter subtype 4 (GLUT4) proteins and glycogen concentration within the quadriceps muscle were assessed. A diabetic rat indicates impaired glucose and insulin tolerances and insulin signalling molecules (IR and GLUT4) proteins and glycogen concentration. Serum insulin, were found to be increased in diabetic rats. The treatment with β-sitosterol normalized the altered levels of blood glucose, serum insulin, and IR and GLUT4 protein levels. Thus, we concluded that β-sitosterol enhances glycemic control through activation of IR and GLUT4 in the quadriceps muscle of high fat diet and sucrose‑induced type2 diabetic rats.
Keywords
β–sitosterol; high fat diet; insulin resistance; IR; GLUT4; type-2 diabetes.
Citation
Sekar et al. Bioinformation 18(11): 1098-1104 (2022)
Edited by
P Kangueane
ISSN
0973-2063
Publisher
License
This is an Open Access article which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited. This is distributed under the terms of the Creative Commons Attribution License.
