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Intrinsically disordered regions in the rodent hepevirus proteome


Zoya Shafat1, Anwar Ahmed2, Mohammad K. Parvez3, Asimul Islam1, Shama Parveen1,*



1Centre for Interdisciplinary Research in Basic Sciences, Jamia Millia Islamia, New Delhi, India; 2Centre of Excellence in Biotechnology Research, College of Science, King Saud University, Riyadh, Saudi Arabia; 3Department of Pharmacognosy, College of Pharmacy, King Saud University, Riyadh, Saudi Arabia; Corresponding author* - Shama Parveen



Shama Parveen - sparveen2@jmi.ac.in; Zoya Shafat - zoya179695@st.jmi.ac.in; Anwar Ahmed - anahmed@ksu.edu.sa; Asimul Islam aislam@jmi.ac.in; Mohammad K Parvez - mohkhalid@ksu.edu.sa


Article Type

Research Article



Received December 8, 2021; Revised January 3, 2022; Accepted January 3, 2022, Published February 28, 2022



Hepatitis E virus (HEV) is the causative agent of Hepatitis E infections across the world. Intrinsically disordered protein regions (IDPRs) or intrinsically disordered proteins (IDPs) are regions or proteins that are characterized by lack of definite structure. These IDPRs or IDPs play significant roles in a wide range of biological processes, such as cell cycle regulation, control of signaling pathways, etc. IDPR/IDP in proteins is associated with the virusís pathogenicity and infectivity. The prevalence of IDPR/IDP in rat HEV proteome remains undetermined. Hence, we examined the unstructured/disordered regions of the open reading frame (ORF) encoded proteins of rat HEV by analyzing the prevalence of intrinsic disorder. The intrinsic disorder propensity analysis showed that the different ORF proteins consisted of varying fraction of intrinsic disorder. The protein ORF3 was identified with maximum propensity for intrinsic disorder while the ORF6 protein had the least fraction of intrinsic disorder. The analysis revealed ORF6 as a structured protein (ORDP); ORF1 and ORF4 as moderately disordered proteins (IDPRs); and ORF3 and ORF5 as highly disordered proteins (IDPs). The protein ORF2 was found to be moderately as well as highly disordered using different predictors, thus, was categorized into both IDPR and IDP. Such disordered regions have important roles in pathogenesis and replication of viruses.



Rat hepevirus; open reading frame (ORF); intrinsic disorder; structured protein; moderately disordered protein; highly disordered protein; ordered protein; intrinsically disordered protein (IDP); intrinsically disordered protein region (IDPR).



Shafat et al. Bioinformation 18(2): 111-118 (2022)


Edited by

P Kangueane






Biomedical Informatics



This is an Open Access article which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited. This is distributed under the terms of the Creative Commons Attribution License.