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Analysis of Bitter honey using gas chromatography and Tandem Mass Spectrometry


Koodathil Joshna*, Venkatachalam Gopal & Baskaran Kavitha



Department of Pharmacognosy, College of Pharmacy, Mother Theresa Post Graduate and Research Institute of Health Sciences, Gorimedu, Puducherry -605006, India; *Corresponding author



Koodathil Joshna - E-mail: joshnak4@gmail.com

Venkatachalam Gopal - E-mail: gopalveni@yahoo.com

Baskaran Kavitha - E-mail: kavimanisep2016@gmail.com  Phone: +91-9789151253


Article Type

Research Article



Received March 3, 2022; Revised March 22, 2022; Accepted March 31, 2022, Published March 31, 2022



Honey has been consumed by humans since ancient times. Honey contains volatile compounds like aldehyde, alcohol, ketone, hydrocarbon, terpenes, acids, benzene compounds. These compounds represent the fingerprint of monofloral honey there by providing information about the floral and geographical origin of honey. The volatile compounds present in honey not only contribute the aroma but also associated with the therapeutic activities of honey. In the present study, the GCMS/MS analysis of bitter honey was carried out to identify the presence of volatile compounds. This is the first study to determine the volatile compounds from ethyl acetate extract of bitter honey produced in the Nilgiri biosphere. Among the eighteen compounds detected, the majority of the compounds were reported to be therapeutically active. Hence further studies regarding the isolation of these compounds could be beneficial in the treatment of various diseases.



Bitter honey, volatile compounds, GCMS/MS



Joshna et al. Bioinformation 18(3): 196-199 (2022)


Edited by

P Kangueane






Biomedical Informatics



This is an Open Access article which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited. This is distributed under the terms of the Creative Commons Attribution License.