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Antimicrobial effects of copper nanoparticles with green tea and neem formulation


Anjali Anna Thomas1 Remmiya Mary Varghese1,* & S Rajeshkumar2



1Department of Orthodontics and Dentofacial Orthopaedics, Saveetha Dental College and Hospital, Saveetha Institute Of Medical and Technical Sciences, Saveetha University, 162, Poonamallee high road, Chennai 600077, Tamilnadu, India; 2Nanobiomedicine Lab, Department of Pharmacology, Saveetha Dental college and Hospital, Saveetha Institute Of Medical And Technical Sciences, Chennai 600077, Tamilnadu, India;*Corresponding author



Anjali Anna Thomas - E-mail: dranjali85400@gmail.com

Remmiya Mary Varghese - E-mail: remmiyavsdc@saveetha.com

S Rajeshkumar - E-mail: ssrajeshkumar@hotmail.com


Article Type

Research Article



Received January 21, 2022; Revised March 31, 2022; Accepted March 31, 2022, Published March 31, 2022



Nanotechnology is the science which is about manipulating matter, atom by atom and is associated with particles smaller than 100 nm in size. Copper nanoparticles are used mainly due to its surplus amount, low cost, easy availability and biocompatible property. Green synthesis of copper nanoparticles is very simple, economical and eco-friendly method that does not involve any toxic chemicals. The aim of our study is green synthesis of copper nanoparticles using green tea and neem formulation and assessment of its antimicrobial effects.20mM of copper sulphate solution is mixed with 40mL of plant extract and 60 mL of distilled water was added and made it into 100 ml solution. Once the copper nanoparticles are synthesized the solution is characterized using UV- vis-spectroscopy and was scanned in double beam UV-vis- spectrophotometer from 300 nm to 700nm wavelength. The antimicrobial property of copper nanoparticle is evaluated by agar well diffusion method. The colour change from green to brown and peak observed in UV-vis- spectrophotometer was associated with the synthesis of copper nanoparticles. Copper nanoparticle from green tea and tea extract has good antimicrobial activity against S.mutans, C.albicans, E.faecalis, & S.aureus. Copper nanoparticles can be efficiently synthesised from green and neem formulation. These copper nanoparticles showed good antibacterial properties and are effective against oral pathogens.



Copper, nanoparticles, neem, green tea, plant extract, antimicrobial activity



Thomas et al. Bioinformation 18(3): 284-288 (2022)


Edited by

P Kangueane






Biomedical Informatics



This is an Open Access article which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited. This is distributed under the terms of the Creative Commons Attribution License.