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Title

Data on antibiotic resistance among indoor microbiome at Meerut, India

Authors

Poonam Rani1, Nouratan Singh2,*, Monika Bhaskar3 & Neeraj Tandan4

 

Affiliation

1Zoology Department, Hansraj College, University of Delhi, India; 2Department of Physiology, UPUMS, Saifai, Etawah- 206130, Uttar Pradesh, India; 3School of Life Sciences, Department of Botany, Guru Ghasidas Vishwavidyalaya, Bilaspur- 495009, Chhattisgarh, India; 4Scientific and Applied Research Center, Post Box No. 2241, Meerut- 250001, U.P., India; *Corresponding Author

 

Email

Poonam Rani - E-mail: poonam@hrc.du.ac.in

Nouratan Singh - E-mail: nouratansingh@gmail.com

Monika Bhaskar - E-mail: monikabhaskar22@gmail.com

Neeraj Tandan - E-mail: neerajtandan@gmail.com

 

Article Type

Research Article

 

Date

Received January 21, 2022; Revised March 31, 2022; Accepted March 31, 2022, Published March 31, 2022

 

Abstract

Microbial dynamics of the domestic environment and their antibiotic-resistant properties have been poorly characterized. We surveyed the microbial community and their antibiotic profiling located in the rural and urban areas of Meerut city, Uttar Pradesh, India. Results show that bacterial community load across all samples had more than 100-fold higher than fungal community (all p<0.05.). Based on population load, the kitchen of both rural (Fungal: 4.161.81 vs Bacteria: 160.527.13) and urban areas (Fungal: 6.21.02 vs Bacteria: 205.4630.9) were more contaminated than living rooms (rural area-Fungal: 2.130.74 vs Bacteria: 62.1720.68 and urban area- Fungal: 4.751.68 vs Bacteria: 74.887.53). Six bacteria, namely Pseudomonas sps; Citrobacter sps; Bacillus Subtilis; Brevundimonas diminuta; Bacillus megaterium; and Klebsiella pneumonia, showed dominance on all other bacterial and fungal sp hence, only these six bacteria were subjected to antibiotic sensitivity test (AST). In AST, Klebsiella pneumoniae, Bacillus Subtilis, Bacillus megaterium, were resistant to more than three antibiotics. The most sensitive strain for Ciprofloxacin, Streptomycin was Citrobacter sp. However, Pseudomonas sp was found sensitive only to Amoxillin. Brevundimonas diminuta is found most sensitive to all antibiotics. Plasmid profiling of selected bacteria suggests that antibiotic resistance properties arose from plasmids, not genomic ones. These findings give new insights into the local-scale distribution of MDR bacteria in a household environment.

 

Keywords

House-hold microbial diversity; bacteria; fungi, antibiotic resistance; plasmid

 

Citation

Rani et al. Bioinformation 18(3): 293-296 (2022)

 

Edited by

P Kangueane

 

ISSN

0973-2063

 

Publisher

Biomedical Informatics

 

License

This is an Open Access article which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited. This is distributed under the terms of the Creative Commons Attribution License.