Title
Antimicrobial, antioxidant and anti-inflammatory activities of seeds from emblica officinalis (Gaertn.)
Authors
Pooja G Singh1,¶, Anisha S Jain1,¶, Poojitha B. Sridhara Setty2, Sushma BV1, Sharanagouda S. Patil3, Ashwini P1, Gopenath TS1, Kuralayanapalya Puttahonnappa Suresh3, Guru Kumar Dugganaboyana1, Karthikeyan Murugesan4, Ashok Gnanasekaran4, Chandan Shivamallu1,*, Shiva Prasad Kollur5, Chandrashekar Srinivasa2, Raghavendra HL6, Parthiban Rudrapathy7,Kanthesh M Basalingappa1,*
Affiliation
1JSS Academy of Higher Education and Research, Mysuru, Karnataka 570 015, India; 2Department of Studies in Biotechnology, Davangere University, Davangere, India; 3ICAR-National Institute of Veterinary Epidemiology and Disease Informatics, Yelahanka, Bengaluru, Karnataka 560 064, India; 4Department of Microbiology, Faculty of Medicine, Quest International University, Ipoh, Perak, Malaysia; 5Department of Sciences, Amrita School of Arts and Sciences, Amrita Vishwa Vidyapeetham, Mysuru Campus, Mysuru, Karnataka 570 026, India; 6Center for Biotechnology, Pravara Institute of Medical Sciences, Loni-413736, Rahata Taluk, Ahmednagar District, Maharashtra, India; 7Malabar Cancer Centre, Department of Clinical Laboratory Services & Translational Research, Thalassery, Kannur 670103, India; *Corresponding authors, ¶Equal contribution & First authorship
Pooja G Singh - E-mail: singhpoojasingh824@gmail.com
Anisha S Jain - E-mail: anishasjain@jssuni.edu.in
Poojitha B. Sridhara Setty - E-mail: bspujitha@gmail.com
Sushma BV - E-mail: sushmabv@jssuni.edu.in
Sharanagouda S. Patil - E-mail: sharanspin13@gmail.com
Ashwini P - E-mail: ashwinip@jssuni.edu.in
Gopenath TS - E-mail: gopenath@jssuni.edu.in
Kuralayanapalya Puttahonnappa Suresh - E-mail: sureshkp97@rediffmail.com
Guru Kumar Dugganaboyana - E-mail: gurukumard@jssuni.edu.in
Karthikeyan Murugesan - E-mail: murugesan.karthikeyan@qiup.edu.my
Ashok Gnanasekaran - E-mail: gnanasekaran.ashok@qiup.edu.my
Chandan Shivamallu - E-mail: chandans@jssuni.edu.in
Shiva Prasad Kollur - E-mail: shivachemist@gmail.com
Chandrashekar Srinivasa - E-mail: chandru.s@davangereuniversity.ac.in
Raghavendra HL - E-mail: raghavendra@pmtpims.org
Parthiban Rudrapathy - E-mail: parthi71975@gmail.com
Kanthesh M Basalingappa - E-mail: kantheshmb@jssuni.edu.in
Article Type
Research Article
Date
Received July 2, 2022; Revised August 31, 2022; Accepted August 31, 2022, Published August 31, 2022
Abstract
There is a shred of evidence to suggest that Emblica officinalis Gaertn, the botanical name for amla seeds, has greater medicinal potential than amla fruit. We conducted this work to assess the anti-inflammatory, antibacterial, and antioxidant capacities of E. officinalis seed extracts. The bioactive components from the seeds were fractionated using chloroform, hexane, methanol, and diethyl ether, according to the polarity of the solvents. The total amount of phenolic and flavonoid was estimated. Both the reducing power and antioxidant capacities of the extracts were evaluated using the DPPH (1,1-diphenyl-2-picryl-hydrazyl) technique. 15-lipoxygenase (LOX) was inhibited by seed extracts at doses ranging from 5 to 25 micrograms. In silico docking was employed to assess the results. Some human pathogenic microorganisms were tested for their antibacterial activity using the agar disc diffusion method. Escherichia coli, Proteus vulgaris, and Klebsiella pneumonia were inhibited by a methanolic extract with an IC50 value of 58g, making it the most common organic solvent extract. Methanolic extracts also showed good antioxidant and antibacterial activity. Our investigation led us to discover that amla seeds have anti-inflammatory, antioxidant, and antibacterial effects.
Keywords
Emblica officinalis; antioxidant; lipoxygenase inhibition; antimicrobial; LC-MS analysis
Citation
Singh et al. Bioinformation 18(8): 683-691 (2022)
Edited by
P Kangueane
ISSN
0973-2063
Publisher
License
This is an Open Access article which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited. This is distributed under the terms of the Creative Commons Attribution License.
