Title
Nano eggshell-titanium-dioxide biocomposite in occluding opened dentine tubules
Authors
Navjot Singh Mann, Ashu Jhamb, Nimrat Kaur* & Navneet Mann
Affiliation
Department of Conservative Dentistry and Endodontics, National Dental College, Derabassi, Mohali, Punjab, India; *Corresponding author; *Corresponding author; Communicated by Anil Kumar
Navjot Singh Mann - E-mail: hodconsendondch@gmail.com
Ashu Jhamb - E-mail: dr_ashu_garg@yahoo.com
Nimrat Kaur - E-mail: nimratkaurkahlon150@gmail.com
Navneet Mann - E-mail: navsandhu727@yahoo.in
Anil Kumar – E-mail: anilkk44@gmail.com
Article Type
Research Article
Date
Received September 2, 2022; Revised October 3, 2022; Accepted October 6, 2022, Published October 31, 2022
Abstract
It is of interest to synthesize Nano eggshell-titanium-dioxide (EB@TiO2) biocomposite and to evaluate its effectiveness in occluding opened dentine tubules. EB@TiO2 was synthesized and characterized using X-ray diffraction (XRD), and Transmission Electron Microscope (TEM). Sixteen simulated bovine dentine discs were prepared and randomly assigned into four groups according to the following treatment (n = 4): Group 1: No treatment; Group 2: eggshell powder; Group 3: EB@TiO2; and Group 4: GIC mousse. These were then, agitated in a solution of 1g powder and 40mL water for 3hours. Thereafter, each dentine discs from the respective groups were post-treated for 5 min with 2wt% citric acid to test their acid resistant characteristics. Scanning Electron Microscope (SEM) was used to observe the effectiveness of occluded dentine pre-treatment and post-treatment. The cytotoxicity of the synthesized EB@TiO2 was tested using NIH 3T3 assay. ANOVA was used to evaluate the mean values of the occluded area ratio and the data of MTS assay. This was followed by a multi-comparison test with Bonferroni correction (α = .05). The XRD confirmed that EB@TiO2 was successfully modified through ball-milling. The TEM revealed the presence of both spherical and irregular particle shape powders. The SEM result showed that EB@TiO2 could effectively occlude open dentine tubules. Equally, the result demonstrated that EB@TiO2 exhibited the highest acid resistant stability post-treatment. NIH 3T3 assay identified that EB@TiO2 had little effect on the NIH 3T3 cell line even at the highest concentration of 100μg/ml. This study suggests that the application of EB@TiO2 effectively occluded dentine tubules and the occlusion showed a high acid resistant stability.
Keywords
Dentin; dentin sensitivity; dentin desensitizing agents; tooth remineralization
Citation
Mann et al. Bioinformation 18(10): 853-857 (2022)
Edited by
P Kangueane
ISSN
0973-2063
Publisher
License
This is an Open Access article which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited. This is distributed under the terms of the Creative Commons Attribution License.
