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Insights from the shotgun-based proteomics analysis of Biomphalaria glabrata


Benson Otarigho1, 2, Mofolusho Falade3,4 & Castro-Borges William5



1Department of Molecular Microbiology and Immunology, Oregon Health and Science University, OR, Portland, USA; 2Department of Biological Science, Edo University,Iyamho, Nigeria; 3Cellular Parasitology Programme, Cell Biology and Genetics Unit, Department of Zoology, University of Ibadan, Ibadan, Nigeria; 4University of Kentucky College of Pharmacy, Lexington, KY, USA; 5Departamento de CiEncias Biologicas, Nucleo de Pesquisas em Ciencias Biologicas, Universidade Federal de Ouro Preto, Ouro Preto, Brazil;



Benson Otarigho Email:Otarigho@ohsu.edu


Article Type

Research Article



Received December 30, 2020; Revised January 30, 2020; Accepted January 31, 2020, Published January 31, 2021



Biomphalaria glabrata is an important host in the transmission of human schistosomiasis in the Caribbean and South America. Therefore, it is of interest to analyse the proteome data of Biomphalaria glabrata hemolymph to identify immunity related proteins in host-pathogen relationship. We used shotgun proteomic and bioinformatic analyses of the non-depleted and depleted [0.5 and 0.75% Trifluoroacetic acid (TFA) depletion] hemolymph of B. glabrata (LE strain). Analysis showed 148 proteins from the hemolymph. 148 were obtained from the 0.5% TFA-depleted sample. 62 proteins follow this from the 0.75% TFA-depleted sample. However, only 59 were found from non-depleted hemolymph. A number of proteins were identified from the hemolymph of this schistosomiasis snail vector linked to immunity related functions. This provides insights to the understanding of schistosome-snail interaction.



Shotgun proteomics; Biomphalaria glabrata; hemolymph; Schistosomiasis; Bioinformatics.



Otarigho et al. Bioinformation 17(1): 266-273 (2021)


Edited by

P Kangueane






Biomedical Informatics



This is an Open Access article which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited. This is distributed under the terms of the Creative Commons Attribution License.