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Chronic inflammation on gingiva-derived mesenchymal stem cells


Anant Ragav Sharma1, Rakesh Kumar Jaiswal2, Swapnali Shinde Kamble3, Mahesh Ghadage4, Madhura Pawar5*, Hiroj Bagde6 & Ramanpal Singh Makkad7,*



1Department of Periodontics, Pacific Dental College, Debari, Udaipur, Rajasthan, India; 2Department of Dentistry, Late Shree Lakhiram Agrawal Memorial Government Medical College Raigarh Chhattisgarh Pin 496001, , India 3Department of Preventive and Pediatric Dentistry, Child Dental Home, Borivali (W), India; 4Department of Prosthodontics and Crown & Bridge.Bharati Vidyapeeth (Deemed to be University) Pune Dental College and Hospital, Navi Mumbai, India; 5Department of Pediatric and Preventive Dentistry, Dr D Y Patil Dental College and Hospital, Dr D Y Patil Vidyapeeth Pimpri, Pune, Maharashtra, India; 6Department of Periodontology, Rama Dental College, Hospital and Research Centre, Kanpur, 7Department of Oral Medicine and Radiology, New Horizon Dental College and Research Institute, Bilaspur, Chhattisgarh, India; *Corresponding author;



Anant Ragav Sharma E-mail: aanantraghavsharma@gmail.com

Rakesh Kumar Jaiswal E-mail: rakeshjaiswal0611@gmail.com

Swapnali Shinde Kamble E-mail: drswapnalishinde@gmail.com

Mahesh Ghadage E-mail: mahesh.ghadage@gmail.com

Madhura Pawar E-mail: drmadhura4@gmail.com

Hiroj Bagde E-mail: hirojbagde8@gmail.com

Ramanpal Singh Makkad E-mail: drramanpal@gmail.com

Vini Mehta - vinip.mehta@gmail.com


Article Type

Research Article



Received January 1, 2023; Revised January 30, 2023; Accepted January 31, 2023, Published January 31, 2023



The impact of inflammatory response on the biological characteristics of GMSCs has been the subject of studies, with conflicting findings. In order to more fully understand the effects of the localized inflammatory environment, the current study assessed the intensity and differentiating capacity of GMSCs derived from healthy periodontal tissues (H-GMSC) and  GMSC derived from inflamed periodontal tissues (I-GMSC) tissues. Cells from every well were taken out and counted using a hemocytometer every three days for a total of 12 days. The findings of the cell proliferation assay, which involved quantifying the cells with the help of a hemocytometer at 0th day, 3rd day, 6th day, and 9th day, are shown. On day nine of culture, there was a considerable (P = 0.02) variation in the rate of multiplication between GMSCs from healthy gingival tissues and GMSCs from gingival tissues having inflammation. Additionally, I-GMSCs had a higher cell concentration on day twelve than that of H-GMSCs. However, there was no significant variance in PDT values comparing GMSCs from healthy gingival tissues and GMSCs from gingival tissues having inflammation (P > 0.05). The mean PDT findings of 66.7 h and 53.4 h have been documented for Healthy-GMSCs and Inflamed-GMSCs, respectively. In addition, compared to GMSCs from healthy gingival tissues, GMSCs from inflammatory tissues had decreased osteogenesis and increased adipogenic potential.  To evaluate the efficacy of GMSCs derived from patients suffering periodontitis utilising human models for cell-based treatments, additional study is necessary.



Chronic inflammation, gingiva-derived mesenchymal stem cells, influence


Sharma et al. Bioinformation 19(1): 138-142 (2023)

Edited by

P Kangueane






Biomedical Informatics



This is an Open Access article which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited. This is distributed under the terms of the Creative Commons Attribution License.