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Title

Molecular biomarkers NOTCH1, CD44, BMI1, and TP53 in oral squamous cell carcinoma

 

Authors

Reshma Mohammad1,*, Anitha Roy2, Rajagopalan Vijayaraghavan1, S. Sreelatha3 & Sarvari Geriki4

 

Affiliation

1Department of Research and Develpoment, Saveetha Institute of Medical and Technical Sciences, Thandalam, Chennai, Tamil Nādu; 2Department of Pharmacology, Saveetha Dental College and Hospitals, Saveetha Institute of Medical and Technical Sciences, Chennai, Tamil Nadu; 3 Department of Anatomy, Malla Reddy Medical College for Women, Hyderabad, Telangana; 4Department of Biochemistry, ACSR Government Medical College, SPSR, Nellore, *Corresponding author

 

Email

Reshma Mohammad E-mail: reshma.svims@gmail.com; Phone: +91 9666935671

Anitha Roy E-mail: anitharoy2015@gmail.com

Rajagopalan Vijayaraghavan E-mail: jai_vijay@hotmail.com

S Sreelatha E-mail: sreelathapoli@gmail.com

Sarvari Geriki E-mail: sarvari.geriki@gmail.com

 

Article Type

Research Article

 

Date

Received May 1, 2023; Revised May 31, 2023; Accepted May 31, 2023, Published May 31, 2023

 

Abstract

It is of interest to evaluate NOTCH1, CD44, BMI1, and TP53 genes in the epiglottis, tongue, and hard palate of oral malignancies (OM) with healthy controls. This was a prospective and cross-sectional study of 60 individuals with oral malignancies (OM) (20 each of tongue, epiglottis, and hard palate) studied at Malla Reddy Medical College and tertiary care hospitals in Hyderabad. Adults aged ≥ 18 years and diagnosed with oral cancer were included in the study. Those who had cancer in more than one area were excluded from the study. Blood samples of individuals with tongue or epiglottis or hard palate were taken for testing the expression of NOTCH1, CD44, TP53, and BMI1 genes. They were analysed by the genomic sequencing method. One-way ANOVA with Bonferroni's t-test was used for statistical analysis.  Expression of   NOTCH1, CD44, BMI1, and TP53 genes were significantly higher in epiglottis, tongue, and hard palate compared to healthy control samples (p < 0.001). All four genes were expressed in all three areas of OM. However, they were not significant between them. Further analysis revealed that NOTCH1, CD44, TP53, and BMI1 genes did not show any difference in HPV-positive and HPV-negative samples. Comparing the T stages of cancer Notch1, gene expression was significantly higher in stages 1 and 2 compared to 3 and 4. The CD44, TP53, and BMI1 did not show any differences in the T stage. However, the difference in HPV in all T stages was very minimal. Data showed that irrespective of the areas of cancer (epiglottis, tongue, and hard palate) NOTCH1, CD44, TP53, and BMI1 genes were expressed equally. The expression was not very much dependent on HPV positive (+ve) or negative (-ve). However the T-stage was showing higher expression compared to control group. Since the expression of these genes was very high in all the three malignancies, they may be used as early biomarkers to detect cancer of epiglottis, tongue, and hard palate.  

 

Keywords

NOTCH1, CD44, BMI1, TP53, human papillomavirus and oral malignancies

 

Citation

Mohammad et al. Bioinformation 19(5): 623-627 (2023)

 

Edited by

P Kangueane

 

ISSN

0973-2063

 

Publisher

Biomedical Informatics

 

License

This is an Open Access article which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited. This is distributed under the terms of the Creative Commons Attribution License.