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Title

Effect of estradiol on histopathology of brain in unilateral and bilateral ovariectomized rats

 

Authors

Vaijayanthimala Ponnusamy1, 2, Kamalakannan Solaiyappan3, Mydhili Govindarasu4, Lavanya Prathap*,5, Shyamaladevi Babu6 & Madhan Krishnan6

 

Affiliation

1Research scholar, Saveetha Medical College, Saveetha Institute of Medical and Technical Sciences (SIMATS), Chennai, Tamilnadu, India; 2Department of Anatomy,Sri Lalithambigai Medical College and hospital, Chennai, Tamilnadu, India; 3Department of Anatomy, Shri Sathya Sai Medical College & Research Institute, Sri Balaji Vidyapeeth (SBV) - Deemed to be University, Ammapettai, Nellikuppam, Chengalpet District, Tamil Nadu 603108; 4Biomedical research unit & laboratory animal centre, Department of Anatomy, Saveetha Dental College and Hospitals, Saveetha Institute of Medical and Technical Sciences (SIMATS), Chennai, Tamilnadu, India; 5Department of Anatomy, Saveetha Dental College and Hospitals, Saveetha Institute of Medical and Technical Sciences (SIMATS), Chennai, Tamilnadu, India; 6Research, Chettinad Hospital and Research Institute, Chettinad Academy of Research and Education, Kelambakkam-603103, Tamil Nadu, India; *Corresponding author

 

Email

Vaijayanthimala Ponnusamy - E-mail:hasinivani2010@gmail.com

Kamalakannan solaiappan - E-mail:anatomykamalakannan@gmail.com

Mydhili Govindarasu E-mail:mydhilig.sdc@saveetha.com

Lavanya Prathap - E-mail:lavanyap.anatomist@gmail.com

Shyamaladevi Babu - E-mail:shyamdevi06@gmail.com; dr.shyamaladevi@care.edu.in

Madhan Krishnan - E-mail:kmadhan91@gmail.com; drmadhan@care.edu.in

 

Article Type

Research Article

 

Date

Received June 1, 2023; Revised June 30, 2023; Accepted June 30, 2023, Published June 30, 2023

 

Abstract

Estradiol, a major form of estrogen, plays a crucial role in various aspects of brain function, including neuronal health, synaptic plasticity, and cognitive processes. Therefore, it is of interest to investigate the histopathological changes of the rat brain following estradiol treatment in unilateral and bilateral ovariectomized rats, a commonly used method to induce estrogen deficiency and study the consequences of hormonal changes. Two months old Wistar albino female rats were divided into five experimental groups, each consisting of 6 animals.  Unilateral and bilateral ovariectomized rats were treated with estradiol (10 mg/Kg/b.wt) subcutaneously for 30 days. The histopathological analysis of brain revealed normal 56 compact layers of small pyramidal cells of CA3 region, most with vesicular nuclei in control rats andan irregular and disturbed thickness of the pyramidal cell layer in the CA3 region, suggesting neuronal loss in unilateral ovariectomized rats. Estradiol therapy to these rats showed dark hyperchromatic layers of pyramidal cells in the CA3 region, most of which displayed vesicular nuclei and less shrinkage. Bilateral ovariectomized rats without estradiol treatment showed irregular and disturbed thickness of the pyramidal cell layer in the CA3 region Similar to Group II, indicating neuronal loss and bilateral ovariectomized rats with estradiol treatment showed no significant changes. These findings highlight the potential role of estradiol in modulating the histopathological changes associated with ovariectomy. Further research is warranted to elucidate the underlying mechanisms through which estradiol exerts its effects on neuronal integrity and to explore potential therapeutic strategies for preventing or reversing these histological changes.

 

Keywords

Ovariectomy; unilateral; bilateral; Brain; Histopathology; Estradiol therapy

 

Citation

Ponnusamy et al. Bioinformation 19(6): 703-706 (2023)

 

Edited by

P Kangueane

 

ISSN

0973-2063

 

Publisher

Biomedical Informatics

 

License

This is an Open Access article which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited. This is distributed under the terms of the Creative Commons Attribution License.