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Genetic polymorphism of Pro12Ala in type 2 diabetes mellitus: Role in inflammation linked to Insulin resistance



Venkata Ramesh Bonam1, Abu Raghavan Srinivasan2*, Daniel Devaprasad Manoj3 & Kudikala Ranjith Babu4



1Department of Biochemistry, Apollo Institute of Medical Sciences and Research (AIMSR), The Apollo University, Chittoor campus, Murukambattu, Chittoor -517127, Andhra Pradesh, India,2Department of Biochemistry, Mahatma Gandhi Medical College & Research Institute, Sri Balaji Vidyapeeth (SBV), SBV Campus, Pillaiyarkuppam, Pondicherry - 607 402, India,3Department of General Medicine, Apollo Institute of Medical Sciences and Research (AIMSR), The Apollo University, Chittoor campus, Murukambattu, Chittoor - 517127, Andhra Pradesh, India,4Department of Physiology, Government Medical College, Rajanna Sircilla, Telangana – 505 301, India.*Corresponding author



Venkata Ramesh Bonam - E-mail: bonam8ramesh@gmail.com & venkataramesh­_b@aimsrchittoor.edu.in

Abu Raghavan Srinivasan - E-mail: raghavan.drvars2000@gmail.com & srinivasanar@mgmcri.ac.in

Daniel Devaprasad Manoj - E-mail:drdanieldmanoj@gmail.com

Kudikala Ranjith Babu - E-mail: drranjithbabu@gmail.com


Article Type

Research Article



Received September 1, 2023; Revised September 30, 2023; Accepted September 30, 2023, Published September 30, 2023



Peroxisome Proliferator-Activated Receptor gamma 2 (PPARγ2) belongs to nuclear receptor superfamily and plays a role in adipocyte differentiation and inflammation. Evidences suggest that inflammatory processes hold key to insulin resistance and PPARγ2 has also been implicated. PPARγ2 exhibits gene polymorphism. The Ala allele of Pro12Ala polymorphism (rs1801282) is associated with a reduced risk for insulin resistance. We attempted the study in overweight and obese males to generate evidences linking insulin resistance, inflammatory mediators, and gene polymorphism of PPARγ2 in overweight and obese males. The conventional biochemical parameters were estimated using established methods. Adiponectin and Haptoglobin were quantitated by ELISA, whereas Ferritin and hs-CRP were by chemi-luminescence. Indices of insulin sensitivity /Insulin resistance were computed based on established formulae. Gene analysis was based on PCR and RFLP. Appropriate statistical analysis was enabled to project gene polymorphism.The heterozygous variant (CG) was around 8 and 38 percent respectively in overweight and obese males. The G Allele was 3.89% and 18.82%. The wild type and heterozygous variant of PPARγ2 depicted significance with haptoglobin, whereas adiponectin showed significance in the wild type. Chi-square test was performed to assess the relation between polymorphic genotypes and ferritin emerged significant. Indices of insulin resistance showed different characteristics with wild type and heterozygous variant ofPPARγ2 gene polymorphism.  The inflammatory mediators (hs-CRP, Ferritin, Haptoglobin and adiponectin) exhibited variegated characteristics with the wild type and heterozygous variant of PPARγ2, thus pointing to the nexus among insulin resistance, inflammation, and adipocyte differentiation.



Genetic polymorphism, Pro12Ala, type 2 diabetes mellitus, inflammation, Insulin resistance



Bonam et al. Bioinformation 19(9): 946-953 (2023)

Edited by

P Kangueane






Biomedical Informatics



This is an Open Access article which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited. This is distributed under the terms of the Creative Commons Attribution License.