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In vitro analysis of material micro-leakage in pit and fissure sealants



Swati Mittal1, Pratyaksha Singh Panwar2, Aabha Rawal3, Mohomedkaif Mansuri4, Anandamoy Bagchi5, Monika Pandey6 & Pratik Surana7,*



1Department of Pedodontics and Preventive Dentistry, Government Dental College and Hospital, Ahmedabad, Gujarat, India;2Department of Dentistry, Government Doon Medical College, Dehradun, Uttarakhand, India;3Department of Conservative Dentistry and Endodontics, Faculty of Dental Science, Dharamsinh Desai University, Nadiad, Gujarat, India;4Department of Public Health Dentistry, Ahmedabad Dental College and Hospital, Ahmedabad, Gujarat, India;5Department of Pedodontics & Preventive Dentistry, Kalinga Institute of Dental Sciences, KIIT Deemed to be University, Bhubaneswar, India;6Department of Paediatric and Preventive Dentistry, R.K.D.F. Dental College and Research Center, Bhopal, Madhya Pradesh, India;7Department of Pedodontics and Preventive Dentistry, Maitri College of Dentistry and Research Centre, Durg, Chhattisgarh, India; *Corresponding author



Swati Mittal - E-mail id: drmittal81@gmail.com; Phone: +91 9909947177

Pratyaksha Singh Panwar - E-mail id: dr.pratyaksha@gmail.com; Phone: +91 9894788183

Aabha Rawal - E-mail id: aabharawal294@gmail.com; Phone: +91 9586762107

Mohomedkaif Mansuri - E-mail id: kaifm4134@gmail.com; Phone: +918849321557

Anandamoy Bagchi - E-mail id: a_bagchi@hotmail.com; Phone: +91 9339536846

Monika Pandey - E-mail id: drmonikapandey25@gmail.com; Phone: +91 9630283879

Pratik Surana - E-mail id: suranadrpratik@gmail.com; Phone: +91 8871310111


Article Type

Research Article



Received April 1, 2024; Revised April 30, 2024; Accepted April 30, 2024, Published April 30, 2024



A preferable choice of material offers superior resistance against micro-leakage for clinical applications in preventing dental caries in pits and fissures is of interest. A total of 45 extracted human premolars were cleaned, stored in a saline solution, and randomly divided into three groups, each intended for treatment with one of the sealants: Fuzi VII, ClinPro, and Embrace Wetbond. The application of the sealants followed the manufacturers' instructions strictly. The teeth were subjected to thermal cycling to simulate oral conditions. Marginal micro-leakage was then assessed by dye penetration method using a 0.5% methylene blue dye. Teeth were sectioned, and dye penetration was measured under a stereomicroscope. The results showed that all the tested materials exhibited some degree of micro-leakage. Within the limitations of this in vitro study, it was concluded that Embrace Wetbond exhibited superior performance in terms of minimizing marginal micro-leakage among the tested pit and fissure sealants.



Pit and fissure sealants, marginal micro-leakage, fuzi vii, clinpro, embrace wetbond



Mittal et al. Bioinformation 20(4): 319-322 (2024)


Edited by

P Kangueane






Biomedical Informatics



This is an Open Access article which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited. This is distributed under the terms of the Creative Commons Attribution License.