HOME   |    PDF   |   


Isolation and characterization of stem cells from human exfoliated deciduous teeth



Ratna Yumkham*,1, C. Nagarathna2, Nelson Sanjenbam3, Angom Gopilal Singh4, Heisnam Philip Singh5 & Albert Ashem6



1Department of Paediatric and Preventive Dentistry, Dental College, RIMS, Imphal, Manipur, India; 2Department of Pedodontics and Preventive Dentistry, RajaRajeswari Dental College and Hospital, Bangalore, Karnataka, India; 3Department of Oral and Maxillofacial Surgery Dental College, JNIMS, Porompat, Imphal, Manipur, India; 4Braces Dental Care and 3D Imaging Centre, Imphal, Manipur, India; 5Orthodontics and Dentofacial Orthopaedics Practioner, Imphal, Manipur, India; 6Department of Oral Medicine and radiology, Dental College, RIMS, Imphal, Manipur, India; *Corresponding author



Ratna Yumkham - E-mail: yumkhamratna@gmail.com; Phone: +91 9366377409

C. Nagarathna - E-mail: shaanrathna@gmail.com; Phone: +91 9845581779

Nelson Sanjenbam - E-mail: sanjenbamnelson@gmail.com; Phone: +91 9366585629

Angom Gopilal Singh - E-mail: bracesdentalcare2017@gmail.com; Phone: +91 9612651893

Heisnam philip Singh - E-mail: philips031@gmail.com; Phone: +91 8258948901

Albert Ashem - E-mail: albertashem@ymail.com; Phone: +91 9612284189


Article Type

Research Article



Received May 1, 2024; Revised May 31, 2024; Accepted May 31, 2024, Published May 31, 2024



SHEDs have been shown to have a higher rate of proliferation and raise in cell population doublings when compared to stem cells from permanent teeth. Hence, using them in tissue engineering may be advantageous over stem cells from adult human teeth. Stem cells were removed from pulpal tissues of thirty primary teeth undergoing extraction under six to fourteen year of age. The tissues were incubated after centrifuging and adding DMEM-KO following the addition of a 2 mg/ml collagenase blend for examination of plates in search of cell attachment and growth. Flow cytometric analysis showed successful isolation of SHEDs using fluoresce inisothiocyanate (FITC)-conjugated CD-34, CD-105, and PE (R-phycoerythrin)-conjugated CD-45, CD-90, CD-73, and HLA-DR antibodies. The surface antigens CD-73, CD-90 and CD-105 which are known to be present in mesenchymal lineages were positively expressed in SHEDs according to flow cytometry analysis, whereas CD-34, CD-45, and HLA-DR were not.



Stem Cells; Multipotent Stem Cells; Dental pulp



Yumkham et al. Bioinformation 20(5): 557-561 (2024)


Edited by

P Kangueane






Biomedical Informatics



This is an Open Access article which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited. This is distributed under the terms of the Creative Commons Attribution License.