Title

Effect of Andrographis paniculata and Melia azadirachta against the Dengue virus

Authors

Kamalraj Mohan¹, KP Gautam Srinivas², Jeevan Malaiyan^3,* & Suresh Arumugam⁴

Affiliation

¹Department of Microbiology, Meenakshi Medical College Hospital & Research Institute, Meenakshi Academy of Higher Education and Research (Deemed to be University), Kanchipuram-631552, Tamilnadu, India; ²Department of General medicine, Meenakshi Medical College Hospital and Research Institute, Meenakshi Academy of Higher Education and Research, TN, India; ³Department of Microbiology, Annaii Medical College Hospital and Research Institute, Pennalur, Sriperumbudur, Chennai-602117, Tamilnadu, India; ⁴Meenakshi Medical College Hospital & Research Institute, Meenakshi Academy of Higher Education and Research (Deemed to be University), Kanchipuram-631552, Tamilnadu, India; *Corresponding author

Email

Kamlaraj Mohan - E - mail: Kamalraj2019@gmail.com
Jeevan Malaiyan - E - mail: jeevan1209@gmail.com
KP Gautam Srinivas - E - mail: gautamcool1993@gmail.com
Suresh Arumugam - E - mail: kpm_suresh@yahoo.com

Article Type

Research Article

Date

Received April 1, 2025; Revised April 30, 2025; Accepted April 30, 2025, Published April 30, 2025

Abstract

Dengue infection is emerging as a global threat due to increased mortality and morbidity, especially in tropical and sub-tropical regions. Therefore, it is of interest to evaluate the antiviral activity of Andrographis paniculata and Melia azadirachta against Dengue virus-1 in a vero cell line using a cytopathic effects (CPE) reduction assay and cytotoxicity assessed by MTT assay. GC-MS analysis was conducted to identify phytochemicals in the plant extracts. Both extracts showed potent antiviral activity at 150 μg/ml and were non-toxic up to 1.5 mg/ml, with 100% cell viability. These findings suggest that A. paniculata and M. azadirachta are promising candidates against Dengue virus-1, warranting further investigation into their active compounds.

Keywords

Dengue, anti-viral activity, cytopathic effects (CPE)

Citation

Mohan et al. Bioinformation 21(4): 730-735 (2025)

Edited by

P Kangueane

ISSN

0973-2063

Publisher

Biomedical Informatics

License

This is an Open Access article which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited. This is distributed under the terms of the Creative Commons Attribution License.