Title

Cytotoxic and apoptotic effects of novel phytochemical extracts on oral squamous cell carcinoma cells

Authors

Sweta Singh¹, Nishita Anthwal², Ankur Joshi³, Rajni Saini⁴, Vipin Arora^5,* & Abhishek Moharana⁶

Affiliation

¹Department of Oral Pathology and Microbiology, Subharti Dental College, Meerut - 250002, India; ²Department of Oral Pathology and Microbiology, Uttaranchal Dental and Medical Research Institute, Haridwar, Majri -Dehradun, Uttarakhand – 248140, India; ³Department of Dentistry, Government Medical College, Haridwar, Uttarakhand – 249401, India; ⁴Department of Oral Pathology and microbiology, Himachal Institute of Dental Sciences, Paonta Sahib, Rampurghat, Sirmaur – 173025, Himachal Pradesh, India; ⁵Department of Restorative Dental Sciences, Taif University, Kingdom of 21944-Saudi Arabia; ⁶Department of Oral Medicine and Radiology, Kalinga Institute of Dental Sciences, Bhubaneswar, KIIT (Deemed to be University), Patia, Bhubaneswar, Odisha, India; *Corresponding author

Email

Sweta Singh - E - mail: shwetasinghtmdcrc@gmail.com
Nishita Anthwal - E - mail: dr.Nishita11@gmail.com
Ankur Joshi - E - mail: drankurbjoshi@gmail.com
Rajni Saini - E - mail: Sainirajni078@gmail.com
Vipin Arora - E - mail: vipinendodontist@gmail.com
Abhishek Moharana - E - mail: guddu17041999@gmail.com

Article Type

Research Article

Date

Received April 1, 2025; Revised April 30, 2025; Accepted April 30, 2025, Published April 30, 2025

Abstract

The oral squamous cell carcinoma has inadequate treatment methods. Therefore, it is of interest to investigate the effect of new phytochemical extracts on both cell death and programmed cell death of OSCC cells. The phytochemical extracts tested at different concentrations produced a decrease in cell survival which dropped from 85% to 40% (p < 0.05) and led to increased apoptotic death from 15% to 60%. Caspase-3 activation tests showed both apoptotic changes and morphological developments at the cellular level for further consideration through in vivo analysis.

Keywords

Oral squamous cell carcinoma (OSCC), phytochemicals, cytotoxicity, apoptosis, caspase-3, anticancer

Citation

Singh et al. Bioinformation 21(4): 801-804 (2025)

Edited by

Hiroj Bagde MDS, (PhD), PGDCR, PGDHHM, PGDL, PGDM

ISSN

0973-2063

Publisher

Biomedical Informatics

License

This is an Open Access article which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited. This is distributed under the terms of the Creative Commons Attribution License.