Title

In vitro evaluation of the anti-microbial efficacy of natural root canal irrigants activated with gamma radiation

Authors

Shrishtee Priya¹, Harleen Kaur Sohi^2,*, Subasish Behera³, Priyanka Pandey⁴, Bibyaswan Chakrabarti⁵, Soumyaranjan Nanda⁶ & Miral Mehta⁷

Affiliation

¹Department of Conservative Dentistry and Endodontics, Vananchal Dental College, Garhwa, Jharkhand, India; ²General Dentist in Massachusetts, 416 Tyler Street, Pittsfield, 01201 Massachusetts, United States; ³Department of Conservative Dentistry and Endodontics, Government Dental College and Hospital (SCB), Manglabag, Cuttack, Odisha, India; ⁴Department of Conservative Dentistry and Endodontics, Bhabha College of Dental Sciences, Bhopal, Madhya Pradesh, India; ⁵Department of Pediatric & Preventive Dentistry, College of Dental Science and Hospital, Rau, Indore, Madhya Pradesh, India; ⁶Department of Endodontics, Private Practitioner, Cuttack, Odisha, India; ⁷Department of Pediatric and Preventive Dentistry, Karnavati School of Dentistry, Karnavati University, Gandhinagar, Gujarat, India; *Corresponding author

Email

Shrishtee Priya - E-mail: shrishteepriya@gmail.com

Harleen Kaur Sohi - E-mail: harleenkaursohi2@gmail.com

Subasish Behera - E-mail: dr.subasish@.gmail.com

Priyanka Pandey - E-mail: priyankasdm1608@gmail.com

Bibyaswan Chakrabarti - E-mail: bittuc27@gmail.com
Soumyaranjan Nanda - E-mail: nandasoumya245@gmail.com
Miral Mehta - E-mail: miral9829@gmail.com

Article Type

Research Article

Date

Received June 1, 2025; Revised June 30, 2025; Accepted June 30, 2025, Published June 30, 2025

Abstract

Neem and Tulsi were investigated as natural root canal irrigants with and without gamma radiation activation to evaluate their performance against Enterococcus faecalis. In total, 60 affected teeth were divided into six groups and each group was treated with a different irrigant. Neem that received gamma-irradiation had the top reduction of bacteria (96.5%), followed by Tulsi and sodium hypochlorite. The effectiveness of non-irradiated extracts was moderate, compared to the mild results found from saline. Gamma radiation made herbal irrigants much more effective, showing their potential for use as biocompatible treatments.

Keywords

Root canal irrigants, natural extracts, gamma radiation, antimicrobial efficacy, enterococcus faecalis, in vitro study

Citation

Priya et al. Bioinformation 21(6): 1471-1474 (2025)

Edited by

Vini Mehta

ISSN

0973-2063

Publisher

Biomedical Informatics

License

This is an Open Access article which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited. This is distributed under the terms of the Creative Commons Attribution License.