HOME   |    PDF   |   


Title

Effect of heavy metal exposure on human kidney cells

 

Authors

Sahil Purushottambhai Patel1, Charu Mishra2, Versha Kumari3, Sanket Patel4, Kirti Nishant Vyas5 & Shruti Dalpatbhai Kalola1,*

 

Affiliation

1Department of Health, GMERS Medical College & Hospital, Vadnagar, Gujarat, India; 2Department of Physiology, Madhav Prasad Tripathi Medical College, Siddharth Nagar, Uttar Pradesh, India; 3Department of Emergency Medicine, Max Hospital, Noida, Uttar Pradesh, India; 4Department of Physiology, GMERS Medical College & Hospital, Vadnagar, Gujarat, India; 5Department of Pathology, Dr N D Desai Faculty of Medical Science and Research, Dharmsinh Desai University, Nadiad, Gujarat, India; *Corresponding author

 

Email

Sahil Purushottambhai Patel - E-mail: sahilpatel150501@gmail.com
Charu Mishra - E-mail: dr.charu423@gmail.com
Versha Kumari - E-mail: vershasingh7.vs@gmail.com
Sanket Patel - E-mail: sanketpatel3028@gmail.com
Kirti Nishant Vyas - E-mail: kirtimdpathology@gmail.com
Shruti Dalpatbhai Kalola - E-mail: shrutikalola0@gmail.com

 

Article Type

Research Article

 

Date

Received June 1, 2025; Revised June 30, 2025; Accepted June 30, 2025, Published June 30, 2025

 

Abstract

Heavy metal cytotoxicity using lab-based cell culture of human kidney (HK-2) cells is of interest. The exposed cells contained lead (Pb), cadmium (Cd) and mercury (Hg) at dosages of 5 μM, 10 μM and 20 μM during incubation periods of 24 hours, 48 hours and 72 hours. Cadmium induced the maximum cell death through its dose-dependent pattern while exposing cells to 20 μM cadmium for 72 hours at 52.4%. Next in line was mercury which reduced cell viability to 60.8% and lead reduced it to 68.9% when cells were exposed to 20 μM for 72 hours. Under cadmium treatment at 20 μM the levels of ROS rose 2.3 times in the target cells. The cells experienced shrinkage of their size and developed membrane blisters through morphological transformations. This study reveals severe kidney damage because cadmium caused the worst impact on renal cell structure.

 

Keywords

Toxicity, kidney cells

 

Citation

Patel et al. Bioinformation 21(6): 1539-1542 (2025)

 

Edited by

Hiroj Bagde MDS, (PhD), PGDCR, PGDHHM, PGDL, PGDM

 

ISSN

0973-2063

 

Publisher

Biomedical Informatics

 

License

This is an Open Access article which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited. This is distributed under the terms of the Creative Commons Attribution License.