Title
Comparative analysis of NAT reactivity and CLIA in detecting transfusion transmitted Infection among blood donors
Authors
Jyoti Kala Bharati, Arvind Kumar Singh, Yatendra Mohan, Aaditya Shivhare, Shweta Chaudhary & Nouratan Singh*
Affiliation
Department of Transfusion Medicine, Uttar Pradesh University of Medical Sciences, Saifai, Etawah, Uttar Pradesh-206130, India; *Corresponding author
Jyoti Kala Bharati - E-mail: jyotikalabharati@gmail.com; jyoti.kala@upums.ac.in
Arvind Kumar Singh - E-mail: arvindsinghjuly001@gmail.com;
arvind.singh@upums.ac.in
Yatendra Mohan - E-mail: yaten.mohan@upums.ac.in;
kumaryatendra1@gmail.com
Aaditya Shivhare - E-mail: aaditya.shiv@upums.ac.in
Shweta Chaudhary - E-mail: shwetakashipur@gmail.com
Nouratan Singh - E-mail: nouratansingh@gmail.com
Article Type
Research Article
Date
Received July 1, 2025; Revised July 31, 2025; Accepted July 31, 2025, Published July 31, 2025
Abstract
The effect of Nucleic Acid Testing (NAT) and Chemiluminescent Immunoassay (CLIA) in detecting TTIs (HIV, HBV, HCV, Syphilis and Malaria by rapid card) among 30,335 blood donations, with a focus on 1,843 reactive units is of interest. NAT showed superior sensitivity (98.50% for HBV, 98% for HIV and 97.50% for HCV) compared to CLIA (94.44.0% for HIV, 79.09% for HBV, 64.20% for HCV), but both methods exhibited high false-positive rates (37.7% for NAT, up to 70.6% for CLIA-HCV). NAT had specificity for HIV (98.5%), HBV (98%) and HCV (98%). CLIA exhibited high false positives (HBV: 27.1%, HCV: 16.5%, HIV: 5.7%), while NAT yield identified 106 HBV (0.35%) and 63 HCV (0.2%) additional cases. NAT was cost-effective for HBV and HCV but less so for HIV. Thus, NAT’s role as a highly sensitive screening tool and with CLIA requiring confirmatory testing to optimize blood supply efficiency is shown.
Keywords
Nucleic acid testing (NAT), Chemiluminescence immunoassay (CLIA), transfusion-transmitted infections (TTIs), blood donors, sensitivity, specificity
Citation
Bharati et al. Bioinformation 21(7): 1947-1951 (2025)
Edited by
P Kangueane
ISSN
0973-2063
Publisher
License
This is an Open Access article which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited. This is distributed under the terms of the Creative Commons Attribution License.
