Title

Exploration of bacteriophage therapy as a viable alternative to combat antibiotic-resistant bacterial infections: A comprehensive in vitro and in vivo evaluation

Authors

Tarang Mehta¹, Saket Verma², Amberpreet Kaur Khangura³, Niva Mahapatra⁴, Khushboo Shrivastava^5,* & Sameer Gupta⁶

Affiliation

¹Department of Oral & Maxillofacial, Pathology and Oral Microbiology, K M Shah Dental College & Hospital, Sumandeep Vidyapeeth Deemed to be University, Vadodara, Gujarat, India; ²Department of Biochemistry, Rajendra Institute of Medical Sciences Ranchi, Jharkhand 834009, India; ³Department of Oral & Maxillofacial Pathology and Oral Microbiology, Dr Harvansh Singh Judge Institute of dental sciences and hospital, Panjab University, Chandigarh, India; ⁴Department of Oral and Maxillofacial Pathology, Kalinga Institute of Dental Sciences, KIIT Deemed to be University, Bhubaneswar, Odisha, India; ⁵Department of Pathology, Nalanda Medical College & Hospital, Patna-800026, Bihar, India; ⁶Department of Oral & Maxillofacial Surgery, Inderprastha Dental College and Hospital, Sahibabad, Ghaziabad, Uttar Pradesh - 201010, India; *Corresponding author

Email

Tarang Mehta - E-mail: tarangmehta111@gmail.com
Saket Verma - E-mail: drsaketverma@gmail.com
Amberpreet Kaur Khangura - E-mail: amber2814@gmail.com
Niva Mahapatra - E-mail: niva.mahapatra@kids.ac.in
Khushboo Shrivastava - E-mail: khushi00691@gmail.com
Sameer Gupta - E-mail: sameer.gupta83@yahoo.in

Article Type

Research Article

Date

Received July 1, 2025; Revised July 31, 2025; Accepted July 31, 2025, Published July 31, 2025

Abstract

Antibiotic resistance in pathogens like E. coli, Klebsiella pneumoniae, and Pseudomonas aeruginosa poses a major threat to global health, with limited treatment options. This study systematically evaluated the efficacy of bacteriophage therapy in vitro and in vivo against multidrug-resistant strains. Phage treatment led to significant bacterial reduction (up to 4.5 log₁₀ CFU/mL) and improved survival rates in mice (up to 85%). Synergistic effects with antibiotics and preservation of gut microbiota were observed without adverse reactions. These findings highlight phage therapy as a promising, targeted, and safe alternative for managing resistant infections.

Keywords

Bacteriophage therapy, multidrug-resistant bacteria, phage-antibiotic synergy

Citation

Mehta et al. Bioinformation 21(7): 2176-2180 (2025)

Edited by

Hiroj Bagde

ISSN

0973-2063

Publisher

Biomedical Informatics

License

This is an Open Access article which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited. This is distributed under the terms of the Creative Commons Attribution License.