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Title

Exploration of bacteriophage therapy as a viable alternative to combat antibiotic-resistant bacterial infections: A comprehensive in vitro and in vivo evaluation

 

Authors

Tarang Mehta1, Saket Verma2, Amberpreet Kaur Khangura3, Niva Mahapatra4, Khushboo Shrivastava5,* & Sameer Gupta6

 

Affiliation

1Department of Oral & Maxillofacial, Pathology and Oral Microbiology, K M Shah Dental College & Hospital, Sumandeep Vidyapeeth Deemed to be University, Vadodara, Gujarat, India; 2Department of Biochemistry, Rajendra Institute of Medical Sciences Ranchi, Jharkhand 834009, India; 3Department of Oral & Maxillofacial Pathology and Oral Microbiology, Dr Harvansh Singh Judge Institute of dental sciences and hospital, Panjab University, Chandigarh, India; 4Department of Oral and Maxillofacial Pathology, Kalinga Institute of Dental Sciences, KIIT Deemed to be University, Bhubaneswar, Odisha, India; 5Department of Pathology, Nalanda Medical College & Hospital, Patna-800026, Bihar, India; 6Department of Oral & Maxillofacial Surgery, Inderprastha Dental College and Hospital, Sahibabad, Ghaziabad, Uttar Pradesh - 201010, India; *Corresponding author

 

Email

Tarang Mehta - E-mail: tarangmehta111@gmail.com
Saket Verma - E-mail: drsaketverma@gmail.com
Amberpreet Kaur Khangura - E-mail: amber2814@gmail.com
Niva Mahapatra - E-mail: niva.mahapatra@kids.ac.in
Khushboo Shrivastava - E-mail: khushi00691@gmail.com
Sameer Gupta - E-mail: sameer.gupta83@yahoo.in

 

Article Type

Research Article

 

Date

Received July 1, 2025; Revised July 31, 2025; Accepted July 31, 2025, Published July 31, 2025

 

Abstract

Antibiotic resistance in pathogens like E. coli, Klebsiella pneumoniae, and Pseudomonas aeruginosa poses a major threat to global health, with limited treatment options. This study systematically evaluated the efficacy of bacteriophage therapy in vitro and in vivo against multidrug-resistant strains. Phage treatment led to significant bacterial reduction (up to 4.5 log₁₀ CFU/mL) and improved survival rates in mice (up to 85%). Synergistic effects with antibiotics and preservation of gut microbiota were observed without adverse reactions. These findings highlight phage therapy as a promising, targeted, and safe alternative for managing resistant infections.

 

Keywords

Bacteriophage therapy, multidrug-resistant bacteria, phage-antibiotic synergy

 

Citation

Mehta et al. Bioinformation 21(7): 2176-2180 (2025)

 

Edited by

Hiroj Bagde, PhD

 

ISSN

0973-2063

 

Publisher

Biomedical Informatics

 

License

This is an Open Access article which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited. This is distributed under the terms of the Creative Commons Attribution License.