Title
Expression of an intron-containing gene from P. falciparum using three sequential PCR steps
Authors
Ameer Abbas, Manoj Yadav, Vikas Kumar & Ankit Gupta*
Affiliation
Department of Biochemistry, All India Institute of Medical Sciences, Raebareli, Uttar Pradesh, India; *Corresponding author
Ameer Abbas - E-mail: abbasameer585@gmail.com
Manoj Yadav - E-mail: manoj.microbiologist56@gmail.com
Vikas Kumar - E-mail: vikaskamalvanshi@gmail.com
Ankit Gupta - E-mail: guptaankitbiochem07@aiimsrbl.edu.in
Article Type
Research Article
Date
Received August 1, 2025; Revised August 31, 2025; Accepted August 31, 2025, Published August 31, 2025
Abstract
The amplification of eukaryotic genes, with introns is very tedious and expensive. The only available lab method is through cDNA synthesis, which has a very low success rate in generating a pure gene fragment. Therefore, it is of interest to devise a strategy to amplify a P. falciparum RPL12mito gene (PfRPL12mito) containing an intron through three sequential PCR steps using genomic DNA (gDNA) and primers with a minimum 15 bp 5'-overhang. The absence of an intronic segment and a continuous exonic region in the amplified gene fragment was confirmed using Sanger sequencing.
Keywords
Intron; Exon; cDNA; gene expression; PCR amplification; P. falciparum
Citation
Abbas et al. Bioinformation 21(8): 2278-2281 (2025)
Edited by
P Kangueane
ISSN
0973-2063
Publisher
License
This is an Open Access article which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited. This is distributed under the terms of the Creative Commons Attribution License.
