Title

In vitro assessment of laser-ablated nanoparticles' cytotoxicity against different oral cancer cells

Authors

Soumendu Bikash Maiti¹*, Pratima Ngangom², Lalit Narayan Singh³, Aaquib Nazir⁴, Manjeet Kumar Shrivastava⁵, Sukhpal Singh⁵, Miral Mehta⁶ & Jugajyoti Pathi⁷

Affiliation

¹Department of Oral Medicine and Radiology, Government Dental College and Hospital, Jamnagar, Gujarat, India; ²Department of Dental, CHC Kakching, Manipur Health Services, Manipur; ³Department of Dentistry, Autonomous State Medical College, Sultanpur, Uttar Pradesh, India; ⁴Department of Dentistry, SNM District Hospital, Leh, UT Ladakh; ⁵Department of Oral Pathology and Microbiology, Divya Jyoti College of Dental Science and Research, Modinagar, Uttar Pradesh, India; ⁶Department of Pediatric and Preventive Dentistry, Karnavati School of Dentistry, Karnavati University, Gandhinagar, Gujarat, India; ⁷Department of Oral & Maxillofacial Surgery, Kalinga Institute of Dental Sciences, KIIT Deemed to be University, Bhubaneswar, Odisha, India; *Corresponding author

Email

Soumendu Bikash Maiti -E-mail: soumendumaiti1985@yahoo.com
Pratima Ngangom - E-mail: pratimangangom7@gmail.com
Lalit Narayan Singh - E-mail: singhlalit347@gmail.com
Aaquib Nazir -E-mail: dr.aaquib.nazir@gmail.com
Manjeet Kumar Shrivastava - E-mail: manjeetshrivastava@gmail.com
Sukhpal Singh -E-mail: drspsingh32@yahoo.com
Miral Mehta -E-mail: miral9829@gmail.com
Jugajyoti Pathi - E-mail: jpathi@kims.ac.in

Article Type

Research Article

Date

Received August 1, 2025; Revised August 31, 2025; Accepted August 31, 2025, Published August 31, 2025

Abstract

Oral cancer is an international health problem that has few treatment methods and a negative prognosis in late cases. The potential cytotoxicity of laser-ablated gold-silver and copper nanoparticles was considered in this study and the effects were tested on oral squamous cell carcinoma cell lines (SCC-4, CAL-27, KB-3-1) and normal human oral keratinocytes (HOK). The most cytotoxic was gold nanoparticles (IC 50: 38.9-45.1 0g/mL) with a marked selectivity (p < 0.001), causing apoptosis, as which flow cytometry and morphological analysis were confirmed. The statistical results obtained were moderate for silver and copper nanoparticles. These results present laser-ablated gold as a potential oral cancer anticancer agent that is specific.

Keywords

Oral squamous cell carcinoma, gold nanoparticles, laser ablation, apoptosis, nanomedicine

Citation

Maiti et al. Bioinformation 21(8): 2393-2397 (2025)

Edited by

Vini Mehta

ISSN

0973-2063

Publisher

Biomedical Informatics

License

This is an Open Access article which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited. This is distributed under the terms of the Creative Commons Attribution License.