Title

Genetic and epigenetic alterations in oral potentially malignant disorders: A cross-sectional clinical study

Authors

Akanksha Baheti¹, Timbadiya Vijaykumar Mansukhbhai^2,*, Parth M Raviya³, Kajal Shilu⁴, Jyoti Singh⁵ & Yadavalli Guruprasad⁶

Affiliation

¹Department of Esthetics and Restorative Dentistry, Associate Dentist, Dental Experts Clinic, Chicago, IL USA; ²Department of Oral Pathology and Microbiology, Rishiraj College of Dental Sciences and Research Centre, Bhopal, Madhya Pradesh, India; ³Department of Oral & Maxillofacial Surgery, Government Dental College and Hospital, Jamnagar, Gujarat, India; ⁴Department of Oral Medicine & Radiology, Government Dental College and Hospital, Jamnagar, Gujarat, India; ⁵Department of Public Health Dentistry, Santosh Dental College, Santosh Deemed to be University, Delhi NCR, India; ⁶Department of Oral & Maxillofacial Surgery, Government Dental College and Research Institute, VIMS Campus, Cantonment, Ballari, Karnataka, India; *Corresponding author

Email

Akanksha Baheti - E-mail: akanshabaheti123@gmail.com
Timbadiya Vijaykumar Mansukhbhai - E-mail: Vijay818nu@gmail.com
Parth M Raviya - E-mail: pmr22389@gmail.com
Kajal Shilu - E-mail: kajalshilu777@gmail.com
Jyoti Singh - E-mail: drjyotisingh134@gmail.com
Yadavalli Guruprasad - E-mail: yadavalliguruprasad@gmail.com

Article Type

Research Article

Date

Received September 1, 2025; Revised September 30, 2025; Accepted September 30, 2025, Published September 30, 2025

Abstract

The prevalence and spectrum of genetic and epigenetic alterations in oral potentially malignant disorders (OPMDs) is of interst. Hence, a total of 132 patients with clinically and histopathologically diagnosed OPMDs were evaluated for key molecular changes, including TP53 mutations, promoter methylation of tumor suppressor genes and global DNA hypomethylation. Salivary and tissue samples were analyzed using PCR, methylation-specific PCR (MSP) and immunohistochemistry. TP53 mutations and p16INK4a promoter hypermethylation were significantly associated with severe dysplasia and higher malignant transformation risk. Thus, integrating molecular profiling into OPMD evaluation could improve risk stratification and early intervention.

Keywords

Oral potentially malignant disorders, genetic alteration, epigenetic modification, TP53, DNA methylation, p16INK4a, oral cancer risk

Citation

Baheti et al. Bioinformation 21(9): 3051-3054 (2025)

Edited by

A Prashanth

ISSN

0973-2063

Publisher

Biomedical Informatics

License

This is an Open Access article which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited. This is distributed under the terms of the Creative Commons Attribution License.