Title
Identification of upregulated genes in pulmonary hypertension using RNA sequencing profiling
Authors
J. Jino Blessy*, R. Jayasurya, Shanmugapriya Murugan & G. Shirley Lois
Affiliation
Department of Bioinformatics, Sri Ramachandra Faculty of Engineering and Technology, Sri Ramachandra Institute of Higher Education and Research, Chennai 600116, India; *Corresponding author
J. Jino Blessy - E-mail: jinoblessy@sriramachandra.edu.in
R. Jayasurya - E-mail:
e8123029@sriher.edu.in
Shanmugapriya Murugan - E-mail: e8124006@sriher.edu.in
G. Shirley Lois - E-mail: b0122053@sriher.edu.in
Article Type
Research Article
Date
Received September 1, 2025; Revised September 30, 2025; Accepted September 30, 2025, Published September 30, 2025
Abstract
Pulmonary hypertension (PH) is a chronic and progressive disease that is characterized by increased pulmonary arterial blood pressure, causing heart strain and eventual heart failure. In the current study PH RNA sequencing (RNA-Seq) dataset involving 8 patients, consisting of 4 cases and 4 controls was selected for analysis. Pathway analysis revealed the involvement of the key genes in pathways crucial for PH. The protein-protein interaction (PPI) network analysis using the STRING database identified key hub genes that were significantly upregulated, including HAUS4, TUBB4A, TUBG1, NEED1, SASS6, NIN, DCUN1D3, CCDC22, ATP2B2 and LRRC37A. These hub gene-encoded proteins can be prominent drug targets for future interventions aimed at treating pulmonary hypertension.
Keywords
Pulmonary hypertension, next-generation sequencing, RNA sequencing, differential gene expression, protein-protein interactions, hub genes
Citation
Blessy et al. Bioinformation 21(9): 3105-3109 (2025)
Edited by
P Kangueane
ISSN
0973-2063
Publisher
License
This is an Open Access article which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited. This is distributed under the terms of the Creative Commons Attribution License.
