Title

Evaluating the presence of aggregatibacter actinomycetemcomitans, porphyromonas gingivalis levels in patient's self- ligating brackets

Authors

Dhananjay Rathod¹, Anukrati Doneria², Subasish Behera³, Soumyaranjan Nanda⁴, Sheetal Acharya⁵, Ananya Bhargava^6,* & Miral Mehta⁷

Affiliation

¹Department of Dentistry, Sheikh Bhikhari Medical College, Hazaribagh, Jharkhand, India; ²Department of Dental Surgery,SN Medical college, Agra, Uttar Pradesh, India; ³Department of Conservative Dentistry and Endodontics, Government Dental College and Hospital (SCB), Manglabag, Cuttack, Odisha, India; ⁴Endodontics, Private Practitioner, Cuttack, Odisha, India; ⁵Department of Periodontics and Implantology, Kalinga Institute of Dental Sciences KIIT (Deemed to be) University, Bhubaneswar, India; ⁶Department of Dentistry, Ruxmaniben deepchand gardi medical college, Ujjain, Madhya Pradesh, India; ⁷Department of Pediatric and Preventive Dentistry, Karnavati School of Dentistry, Karnavati University, Gandhinagar, Gujarat, India; *Corresponding author

Email

Dhananjay Rathod - E-mail: Drdhananjayrathod@gmail.com
Anukrati Doneria - E-mail: doneria.anu21@gmail.com
Subasish Behera - E-mail: dr.subasish@gmail.com
Soumyaranjan Nanda - E-mail: nandasoumya245@gmail.com
Sheetal Acharya - E-mail: sheetal.acharya@kids.ac.in
Ananya Bhargava - E-mail: drananyaortho@gmail.com
Miral Mehta - E-mail: miral9829@gmail.com

Article Type

Research Article

Date

Received September 1, 2025; Revised September 30, 2025; Accepted September 30, 2025, Published September 30, 2025

Abstract

The impact of self-ligating brackets on periodontal health remains uncertain, particularly regarding colonization by key pathogens such as Aggregatibacter actinomycetemcomitans and Porphyromonas gingivalis. Therefore, it is of interest to evaluate microbial changes in 40 orthodontic patients fitted with self-ligating brackets. Subgingival plaque samples were collected at baseline, 1 month and 3 months and analyzed using real-time PCR. Results showed a significant rise in P. gingivalis and a moderate increase in A. actinomycetemcomitans, with the highest accumulation observed around posterior brackets. Thus, we show that self-ligating brackets may favor pathogen colonization, highlighting the importance of strict oral hygiene measures to reduce periodontal risks.

Keywords

Self-ligating brackets, Aggregatibacter actinomycetemcomitans, Porphyromonas gingivalis, orthodontic microbiology, periodontal pathogens, RT-PCR.

Citation

Rathod et al. Bioinformation 21(9): 3235-3238 (2025)

Edited by

Hiroj Bagde

ISSN

0973-2063

Publisher

Biomedical Informatics

License

This is an Open Access article which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited. This is distributed under the terms of the Creative Commons Attribution License.