Title
Molecular docking analysis of PI3K/AKT with betulin from Impatiens henslowiana
Authors
Rajamathanky Hariharan & Rajasekaran Aiyalu*
Affiliation
Department of Pharmacology, KMCH College of Pharmacy, Coimbatore, Tamil Nadu, India; *Corresponding author
Rajamathanky Hariharan - E-mail: rajamathanky.kmch@gmail.com
Rajasekaran Aiyalu - E-mail: aiyalu.kmch@gmail.com
Article Type
Research Article
Date
Received September 1, 2025; Revised September 30, 2025; Accepted September 30, 2025, Published September 30, 2025
Abstract
Drug-induced liver injury (DILI) remains a major clinical concern, as current therapies often show low efficacy and undesirable side effects. This study investigated the hepatoprotective, antioxidant, and anti-inflammatory potential of Impatiens henslowiana (IH) ethanolic extract and its active compound, betulin. IH was non-toxic up to 4000 mg/kg and showed protective effects in D-GalN-induced hepatotoxic rats at 200 and 400 mg/kg. Betulin exhibited strong antioxidant activity at a concentration of 9.5 μM and 19 μM in DCFH-DA assay, along with cytoprotective activity and downregulation of PI3K and AKT expression and their phosphorylation in HepG2 cells. Molecular docking confirmed high binding affinities of betulin to PI3K and AKT with binding energies of –10.36 kcal/mol and –10.99 kcal/mol, respectively, supporting its therapeutic potential.
Keywords
Impatiens henslowiana, betulin, hepatoprotective, anti-inflammatory, antioxidant, PI3K/AKT signaling
Citation
Hariharan & Aiyalu, Bioinformation 21(9): 3269-3282 (2025)
Edited by
P Kangueane
ISSN
0973-2063
Publisher
License
This is an Open Access article which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited. This is distributed under the terms of the Creative Commons Attribution License.
