Title

Potential use of liquid biopsy in diagnosing oral malignancies

Authors

Timbadiya Vijaykumar Mansukhbhai¹, Madhvika Patidar^2,*, Manjiri Chakor³, Dakshayani Vijay Patil⁴, Pawan Rebello⁵, Gatha Mohanty⁶ & Rashmi Laddha⁷

Affiliation

¹Department of Oral Pathology and Microbiology, Rishiraj College of Dental Science and Research Centre, Bhopal, Madhya Pradesh, India; ²Department of Oral Pathology and Microbiology, Government College of Dentistry, Indore, Madhya Pradesh, India; ³Department of Oral and Maxillofacial Surgery, RD Dental College & Research Center, Nagpur, Maharashtra, India; ⁴Department of Oral & Maxillofacial Pathology, Hazaribag College of Dental Sciences and Hospital, Hazaribagh, Jharkhand, India; ⁵Department of Oral & Maxillofacial Pathology & Oral Microbiology, Sardar Patel Postgraduate Institute of Dental and Medical Sciences, Lucknow, Uttar Pradesh, India; ⁶Department of Periodontics and Oral Implantology, Institute of Dental Science, Siksha 'O' Anusandhan Deemed to be University, Bhubaneswar, Odisha, India; ⁷Department of Periodontology, RR Kambe Dental College and Hospital, Akola, Maharashtra, India; *Corresponding author

Email

Timbadiya Vijaykumar Mansukhbhai - E-mail: vijay818nu@gmail.com
Madhvika Patidar - E-mail: madhvikapatidar@gmail.com
Manjiri Chakor - E-mail: chakormanjiri@gmail.com
Dakshayani Vijay Patil - E-mail: drdakshayanipatil@gmail.com
Pawan Rebello - E-mail: pawanrebello@gmail.com
Gatha Mohanty - E-mail: gathamohanty@soa.ac.in
Rashmi Laddha - E-mail: drrashmirdaga@gmail.com

Article Type

Research Article

Date

Received October 1, 2025; Revised October 31, 2025; Accepted October 31, 2025, Published October 31, 2025

Abstract

Liquid biopsy is a minimally invasive method with strong potential for detecting and monitoring oral cancers. By analyzing ctDNA, CTCs, exosomes and other tumor-derived biomarkers, it enables early detection, prognosis and treatment monitoring. Unlike tissue biopsy, it allows repeated sampling to track tumor burden and genetic changes over time. In oral squamous cell carcinoma, studies show good sensitivity and specificity for detecting mutations, methylation and protein markers. However, standardization, biomarker validation and cost–effectiveness remain challenges for clinical use.

Keywords

Liquid biopsy; oral malignancies; circulating tumor DNA; circulating tumor cells; exosomes; early cancer detection; non-invasive diagnostics; oral squamous cell carcinoma

Citation

Mansukhbhai et al. Bioinformation 21(10): 3465-3469 (2025)

Edited by

A Prashanth

ISSN

0973-2063

Publisher

Biomedical Informatics

License

This is an Open Access article which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited. This is distributed under the terms of the Creative Commons Attribution License.