Title

Effect of RF and Anti-CCP antibody for Rheumatoid Arthritis diagnosis: A comparative study

Authors

Rohit Dubepuria¹, Dinesh Singh Mahor², Shubham Gupta¹ & Nishtha Jain^3,*

Affiliation

¹Department of General Medicine, Chirayu Medical College & Hospital, Bhopal, Madhya Pradesh, India; ²Department of General Medicine, Government Medical College, Sheopur, Madhya Pradesh, India; ³Department of General Medicine, R.D Gardi Medical College, Ujjain, Madhya Pradesh, India; *Corresponding author

Email

Rohit Dubepuria - E-mail: rohit_0122@rediffmail.com
Dinesh Singh Mahor - E-mail: dr.dineshmahor@gmail.com
Shubham Gupta - E-mail: docsgupta9931@gmail.com
Nishtha Jain - E-mail: nishtha1925@gmail.com

Article Type

Research Article

Date

Received November 15, 2025; Revised December 15, 2025; Accepted December 15, 2025, Published December 15, 2025

Abstract

Rheumatoid arthritis (RA) is a long-term autoimmune disease that needs to be diagnosed as soon as possible in order to achieve maximum treatment results. Although rheumatoid factor (RF) has been a conventional serologic test, the anti-cyclic citrullinated peptide (anti-CCP) antibodies are now considered a more specific diagnostic test. Therefore, it is of interest to determine the diagnostics performance of RF and anti-CCP antibody tests in suspected RA patients in a tertiary care hospital. One hundred and eighty participants will be recruited, that is, 120 participants diagnosed with RA clinically (according to the 2010 ACR/EULAR definitions) and 60 age-matched controls. RF in serum samples was studied by nephelometry and the anti-CCP antibodies through enzyme-linked immunosorbent assay (ELISA). Anti-CCP antibodies were more specific (96.7% vs. RF, 78.3%), whereas the sensitivity was also similar (81.7% vs. 75.0% respectively). Anti-CCP (96.1) had a better positive predictive value than RF (85.7%). The sensitivity (88.3%) and specificity (95.0%) with combined testing were improved. Anti-CCP was found to have significant correlations with disease activities and radiological erosions. Data shows uphold anti-CCP antibodies as a better diagnostic tool than RF in Diagnosing RA and the combination of the two tests provides the best diagnostic value in the early stages of the disease and prognostication.

Keywords

Rheumatoid arthritis, Anti-CCP antibodies, rheumatoid factor, diagnostic accuracy, autoimmune disease

Citation

Dubepuria et al. Bioinformation 21(12): 4420-4424 (2025)

Edited by

Vini Mehta

ISSN

0973-2063

Publisher

Biomedical Informatics

License

This is an Open Access article which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited. This is distributed under the terms of the Creative Commons Attribution License.