Title
Antimicrobial activity of probiotic irrigant with and without mentha piperata essential oil against E. Faecalis: An in vitro comparative study
Authors
Anuja Ajaykumar Dhumal1,*, Damini Patil2, Akshay Arun Khase3, Priyanka Dhananjay Kombade4, Bhagyashri Narayan Magar5 & Yogesh Balaji Chanchalwad3
Affiliation
1Department of Conservative Dentistry and Endodontics, Bharati Vidyapeeth Deemed to be University Dental College and Hospital, Sangli, Maharashtra, India; 2Department of Conservative Dentistry and Endodontics, Y.M.T. Dental College, Kharghar, Navi Mumbai, Maharashtra, India; 3Department of Conservative Dentistry and Endodontics, MIDSR, Latur, Maharashtra, India; 4Department of Conservative Dentistry and Endodontics, SB Patil Dental College and Hospital, Bidar, Karnataka, India; 5Department of Conservative Dentistry and Endodontics, School of Dental Sciences Krishna Vishwa Vidyapeeth, Malkapur, Karad, Maharashtra, India; *Corresponding author
Anuja Ajaykumar Dhumal - E-mail: anuja.dhumal@bharatividyapeeth.edu.in
Damini Patil - E-mail: damininote2@gmail.com
Akshay Arun Khase - E-mail: akshaykhase68@gmail.com
Priyanka Dhananjay Kombade - E-mail: priyankakombade03@gmail.com
Bhagyashri Narayan Magar - E-mail: dr.bhagyashrimagar@gmail.com
Yogesh Balaji Chanchalwad - E-mail: yogeshchanchalwad1409@gmail.com
Article Type
Research Article
Date
Received January 1, 2026; Revised January 31, 2026; Accepted January 31, 2026, Published January 31, 2026
Abstract
Persistent Enterococcus faecalis infections in root canals following endodontic therapy pose significant treatment challenges due to its antimicrobial resistance and biofilm formation. Therefore, it is of interest to evaluate the antimicrobial activity of probiotic irrigant (Lactobacillus rhamnosus) with and without mentha piperata essential oil against E. faecalis by measuring zones of inhibition on Mueller Hinton agar and blood agar plates at 24- and 48-hour intervals. E. faecalis was cultured on agar plates, and three irrigant solutions were tested: 5.25% sodium hypochlorite (Group 1), probiotic irrigant alone (Group 2) and probiotic irrigant with mentha piperata essential oil (Group 3). Data shows that probiotic irrigant combined with mentha piperata essential oil (Group 3) exhibited significantly larger zones of inhibition compared to sodium hypochlorite alone (Group 1) on both media, while probiotic irrigant without essential oil (Group 2) showed comparable inhibition to sodium hypochlorite. Probiotic irrigant combined with Mentha piperita essential oil demonstrates superior antimicrobial efficacy against E. faecalis, with recommendations for future in vivo studies to evaluate effectiveness under complex oral conditions.
Keywords
Probiotic irrigant, mentha piperata essential oil, E.faecalis, Lactobacillus rhamnosus
Citation
Dhumal et al. Bioinformation 22(1): 186-191 (2026)
Edited by
Vini Mehta
ISSN
0973-2063
Publisher
License
This is an Open Access article which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited. This is distributed under the terms of the Creative Commons Attribution License.
