Title

Comparative evaluation of remineralisation potential using BioHAP, CPP-ACP and Enamelon on root dentin: An in vitro study

Authors

Rashmi Patil^1,*, Rinky Hajong², Aslam Tariq Mohammed³, Sruthi Keerthi⁴, Pranoti Lavekar⁵, Dimple Rakhecha⁶, Rubeena Naaz⁷ & Md Kafeel Ahmed⁸

Affiliation

¹Department of Conservative and Endodontics, P.M.N.M Dental College and Hospital, Bagalkot, Karnataka, India; ²Department of Conservative Dentistry and Endodontics, Government Dental College and Hospital, Silchar, Assam, India; ³Department of Orthodontics, Narayana Dental College, Nellore andhra Pradesh, India; ⁴Pedodontics and Preventive Dentistry, Consultant in Hyderabad, Telangana, India; ⁵Department of Public Health Dentistry, Private Practitioner, Pune, Maharashtra, India; ⁶Department of Conservative Dentistry and Endodontics, Government Dental College and Hospital, Ahmedabad, Gujarat, India; ⁷Dental SurgeonSRK Dental Clinic, Hyderabad, Telangana, India; ⁸Department of Periodontology and Implantology, MNR Dental College and Hospital, Sangareddy, Hyderabad, Telangana, India; *Corresponding author

Email

Rashmi Patil - E-mail: drrashmipatil11@gmail.com; Phone: +91 99000 04208
Rinky Hajong - E-mail: rinkyhajong123@gmail.com; Phone: +91 60000 17409
Aslam Tariq Mohammed - E-mail: draslamtariq@gmail.com; Phone: +96 565789143
Sruthi Keerthi - E-mail: sruthikeerthi.s@gmail.com; Phone: +91 7738607997
Pranoti Lavekar - E-mail: pranotilavekar7768@gmail.com; Phone: +91 9834322709
Dimple Rakhecha - E-mail: rakhechadimple.dr@gmail.com; Phone: +91 8758617639
Rubeena Naaz - E-mail: rubeenakhan412@gmail.com; Phone: +91 9980556623
Md Kafeel Ahmed - E-mail: drkafeelsohar@gmail.com; Phone: +91 9880585623

Article Type

Research Article

Date

Received March 1, 2026; Revised March 31, 2026; Accepted March 31, 2026, Published March 31, 2026

Abstract

Root caries and cervical dentin hypersensitivity affect older adults with gingival recession, requiring effective remineralisation strategies for root dentin preservation. Therefore, it is of interest to compare remineralisation potential of BioHAP, CPP-ACP and Enamelon on artificially demineralised root dentin from 60 extracted premolars using a 28-day pH cycling protocol. All agents significantly increased microhardness versus control (p<0.001), with BioHAP achieving superior recovery at 68.42±7.83% compared to other groups. SEM analysis confirmed BioHAP's enhanced mineral deposition and dentinal tubular occlusion, demonstrating superior structural repair capabilities. These findings advance restorative dentistry by establishing BioHAP as the optimal commercial agent for root dentin remineralisation and hypersensitivity management.

Keywords

Root dentin, remineralisation, biomimetic hydroxyapatite (BioHAP), casein phosphopeptide-amorphous calcium phosphate (CPP-ACP), enamelon

Citation

Patil et al. Bioinformation 22(3): 1339-1345 (2026)

Edited by

Vini Mehta

ISSN

0973-2063

Publisher

Biomedical Informatics

License

This is an Open Access article which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited. This is distributed under the terms of the Creative Commons Attribution License.