Title

Biological and toxicological evaluation of chicken eggshell membrane for guided bone regeneration: An in vitro assessment

Authors

Deepak Chandrasekaran¹, Sharada T Rajan^2,*, Ravindran Chinnaswami¹ & Nadathur
Doraiswamy Jayakumar³

Affiliation

¹Department of Oral and Maxillofacial Surgery, Sri Ramachandra Dental College and Hospital, SRIHER, Chennai, India; ²Department of Oral & Maxillofacial Pathology, Sri Ramachandra Dental College & Hospital, SRIHER, Chennai, India; ³Department of Periodontics & Implantology, Saveetha Dental College, Chennai, India; *Corresponding author

Email

Deepak Chandrasekaran - E-mail: deepakc@sriramachandra.edu.in; Phone: +91 9840261543
Sharada Thiaga Rajan - E-mail: sharadarajan@sriramachandra.edu.in; Phone: +91 9840082472
Ravindran Chinnaswami - E-mail: ravindran642@yahoo.com; Phone: +91 9841030237
Nadathur Doraiswamy Jayakumar - E-mail: profndj@yahoo.com; Phone: +91 9444071930

Article Type

Research Article

Date

Received March 1, 2026; Revised March 31, 2026; Accepted March 31, 2026, Published March 31, 2026

Abstract

The lack of cost-effective, biocompatible and sustainable barrier membranes remains a significant limitation in guided bone regeneration (GBR). Therefore, it is of interest to evaluate the biological compatibility and toxicological safety of chicken eggshell membrane as a potential GBR barrier membrane using standardized in vitro assays. Physical characterization, cell viability, proliferation, cytotoxicity grading and toxicological assessments were performed under controlled laboratory conditions. The membrane demonstrated high cell viability (>94%), progressive proliferation, non-cytotoxic classification and absence of inflammatory or necrotic responses. Thus, we show that chicken eggshell membrane exhibits excellent biocompatibility with minimal toxicological risk, supporting its potential as a sustainable alternative membrane for GBR applications.

Keywords

Guided bone regeneration (GBR); eggshell membrane; barrier membrane; biocompatibility; toxicological evaluation; biomaterials; collagen-based membrane; bone tissue engineering

Citation

Chandrasekaran et al. Bioinformation 22(3): 1915-1918 (2026)

Edited by

A Prashanth

ISSN

0973-2063

Publisher

Biomedical Informatics

License

This is an Open Access article which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited. This is distributed under the terms of the Creative Commons Attribution License.