Title
Biological and toxicological evaluation of chicken eggshell membrane for guided bone regeneration: An in vitro assessment
Authors
Deepak Chandrasekaran1, Sharada Thiaga Rajan1,*, Ravindran Chinnaswami1 & Nadathur Doraiswamy Jayakumar2
Affiliation
1Department of Oral & Maxillofacial Surgery, Sri Ramachandra Dental College & Hospital, SRIHER, Sri Ramachandra Nagar, Iyyappanthangal, Chennai, Tamil Nadu 600116, India; 2Department of Periodontics & Implantology, Saveetha Dental College, Poonamallee High Rd, Velappanchavadi, Chennai, Tamil Nadu 600077, India; *Corresponding author
Deepak Chandrasekaran - E-mail: deepakc@sriramachandra.edu.in;
Phone: +91 9840261543
Sharada Thiaga Rajan - E-mail: sharadarajan@sriramachandra.edu.in;
Phone: +91 9840082472
Ravindran Chinnaswami - E-mail: ravindran642@yahoo.com; Phone: +91
9841030237
Nadathur Doraiswamy Jayakumar - E-mail: profndj@yahoo.com; Phone:
+91 9444071930
Article Type
Research Article
Date
Received March 1, 2026; Revised March 31, 2026; Accepted March 31, 2026, Published March 31, 2026
Abstract
The lack of cost-effective, biocompatible and sustainable barrier membranes remains a significant limitation in guided bone regeneration (GBR). Therefore, it is of interest to evaluate the biological compatibility and toxicological safety of chicken eggshell membrane as a potential GBR barrier membrane using standardized in vitro assays. Physical characterization, cell viability, proliferation, cytotoxicity grading and toxicological assessments were performed under controlled laboratory conditions. The membrane demonstrated high cell viability (>94%), progressive proliferation, non-cytotoxic classification and absence of inflammatory or necrotic responses. Thus, we show that chicken eggshell membrane exhibits excellent biocompatibility with minimal toxicological risk, supporting its potential as a sustainable alternative membrane for GBR applications.
Keywords
Guided bone regeneration (GBR); eggshell membrane; barrier membrane; biocompatibility; toxicological evaluation; biomaterials; collagen-based membrane; bone tissue engineering
Citation
Chandrasekaran et al. Bioinformation 22(3): 1915-1918 (2026)
Edited by
P Kangueane
ISSN
0973-2063
Publisher
License
This is an Open Access article which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited. This is distributed under the terms of the Creative Commons Attribution License.
